The promoter and transcriptional unit of a novel herpes simplex virus 1 alpha gene are contained in, and encode a protein in frame with, the open reading frame of the alpha 22 gene
The promoter and transcriptional unit of a novel herpes simplex virus 1 alpha gene are contained in, and encode a protein in frame with, the open reading frame of the alpha 22 gene
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DOI：
10.1128/jvi.70.1.172-178.1996
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发表时间：
1996-01-01
影响因子：
5.4
通讯作者：
Roizman, B
中科院分区：
文献类型：
--
作者：
Carter, KL;Roizman, B
单纯疱疹病毒1型基因组编码一组基因（α基因），在没有新生病毒蛋白合成的情况下表达，早期的研究表明，α 22基因的产物被酪蛋白激酶II核苷酸化，并被U(L)13和U(S)3编码的病毒蛋白激酶磷酸化。缺乏以氨基酸200开头的羧基末端结构域的突变体在原代人类细胞株或啮齿动物衍生的细胞中表现出复制能力降低，并且还表现出γ或Late基因子集的表达减少。我们报道了α 22基因的结构域由两个3'-共端mrna转录。较长的转录本编码420个氨基酸的α 22蛋白，而较短的转录本编码含有α 22蛋白羧基末端273个氨基酸的蛋白质，较短的基因被命名为U(S)1.5。U(S)1.5 mRNA在感染细胞中合成，并在环己亚胺存在和其他限制病毒基因表达为α基因的条件下维持。在α 22蛋白密码子200或240后插入编码18、21或22个氨基酸的连接子不会影响与野生型α 22基因或其产物相关的已知功能或表型，早期的研究将核苷酸化序列置于蛋白质的氨基末端部分。这些研究结果表明，U(S)1.5基因编码与人类原代或啮齿动物细胞复制相关的功能，以及α 0和γ基因的最佳表达，这一发现使已知在1型单纯疱疹病毒DNA独特短区域定位的基因数量达到14个，不同基因的总数达到78个。
The herpes simplex virus type 1 genome encodes a set of genes (alpha genes) expressed in the absence of de novo viral protein synthesis, Earlier studies have shown that the product of the alpha 22 gene, a member of this set, is nucleotidylylated by casein kinase II and phosphorylated by viral protein kinases encoded by U(L)13 and U(S)3. Mutants lacking the carboxyl-terminal domain starting with amino acid 200 exhibit reduced capacity to replicate in primary Human cell strains or in cells of rodent derivation and also exhibit reduced expression of a subset of gamma or Late genes. We report that the domain of the alpha 22 gene is transcribed by two 3'-coterminal mRNAs. The longer transcript reported encodes the 420-amino-acid alpha 22 protein, whereas the shorter transcript reported here encodes a protein containing the carboxyl-terminal 273 amino acids of the alpha 22 protein, The shorter gene is designated U(S)1.5. The U(S)1.5 mRNA is synthesized in cells infected and maintained in the presence of cycloheximide and under other conditions which restrict viral gene expression to alpha genes. In-frame insertion of linkers encoding 18, 21, or 22 amino acids after codon 200 or 240 of the alpha 22 protein did not effect the known functions or phenotype associated with the wild-type alpha 22 gene or its product, Earlier studies have placed the nucleotidylylated sequences in the amino-terminal portion of the protein, The results of these studies indicate that the U(S)1.5 gene encodes the functions associated with replication in human primary or rodent cells and optimal expression of alpha 0 and gamma genes, This finding brings the number of genes known to map in the unique short region of the herpes simplex virus type 1 DNA to 14 and the total number of different genes to 78.