Injectable thermo-sensitive hydrogel containing ADSC-derived exosomes for the treatment of cavernous nerve injury
Injectable thermo-sensitive hydrogel containing ADSC-derived exosomes for the treatment of cavernous nerve injury
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含有 ADSC 衍生外泌体的可注射热敏水凝胶用于治疗海绵体神经损伤
DOI：
10.1016/j.carbpol.2022.120226
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发表时间：
2022-10-27
影响因子：
11.2
通讯作者：
Fu, Qiang
中科院分区：
文献类型：
--
作者：
Liu, Shuai;Li, Ruiyu;Fu, Qiang
根治性盆腔手术通常伴有海绵体神经损伤（CNI-ED）引起的术后勃起功能障碍的风险。使用脂肪间充质干细胞衍生的外泌体（ADSC-Exo）治疗神经退行性疾病的策略已显示出有希望的结果。然而，延长未结合的ADSC-Exo在受损组织中的保留以发挥治疗效果仍然具有挑战性。本研究开发了一种新型温敏性羟乙基壳聚糖/β-甘油磷酸钠水凝胶（HG@Exo），用于治疗CNI-ED，具有良好的注射性、结构稳定性和体温敏感性。体内评估表明，所设计的负载ADSC-Exo的HG水凝胶增强了ADSC-Exo的保留并显示出缓慢释放。此外，当HG@Exo应用于神经损伤部位时，双侧海绵体神经损伤大鼠模型的勃起功能明显改善。因此，我们的研究结果表明，开发的生物活性水凝胶提出了一个有前途的战略，有效的管理CNI-ED。
Radical pelvic surgery is commonly accompanied by the risk of postoperative erectile dysfunction induced by cavernous nerve injury (CNI-ED). The strategy of using adipose mesenchymal stem cell-derived exosomes (ADSC-Exo) to treat neurodegenerative diseases has shown promising results. However, it remains challenging to prolong the retention of unbound ADSC-Exo in damaged tissues to exert therapeutic effects. Herein, we develop a novel injectable thermo-sensitive hydroxyethyl chitosan/sodium beta-glycerophosphate hydrogel (HG) encapsu-lating ADSC-Exo (HG@Exo) to manage CNI-ED. The HG exhibits excellent injectability, structural stability, and body temperature sensitivity. In vivo assessment demonstrates that the designed ADSC-Exo-loaded HG hydrogel enhances the retention of ADSC-Exo and displays a slow release. Furthermore, when HG@Exo is applied to the site of nerve injury, erectile function in the bilateral cavernous nerve injury rat model is significantly improved. Thus, our finding indicates that the developed bioactive hydrogel presents a promising strategy for the effective management of CNI-ED.