INTERACTION OF L-PNEUMOPHILA AND A FREE LIVING AMEBA (ACANTHAMOEBA, PALESTINENSIS)
INTERACTION OF L-PNEUMOPHILA AND A FREE LIVING AMEBA (ACANTHAMOEBA, PALESTINENSIS)
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DOI：
10.1017/s0022172400060174
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发表时间：
1983-01-01
影响因子：
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通讯作者：
KURTZ, JB
中科院分区：
文献类型：
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作者：
ANAND, CM;SKINNER, AR;KURTZ, JB
L.嗜肺菌血清群I和A.在Neff ′ s培养基中，在35 ℃下，电镜观察和免疫荧光分析表明，C能促进细菌的细胞内增殖。在使用的封闭实验系统中，军团菌的数量从107个菌落形成单位（c.f.u.）/ ml初始至最大1010 c.f.u./ ml，第5天。在排出的吞噬体中发现军团菌，在一些充满细胞质的变形虫中发现军团菌，在另一些变形虫中发现包囊形成过程似乎已经开始。在20度。棘阿米巴吞噬并消化军团菌。到第2天，细菌从共培养瓶中消失，但以低数量（102 c.f.u./ ml），表明即使在该温度下也发生了一些阿米巴内增殖。
Co-cultivation of L. pneumophila serogroup I and A. palestinensis in Neff''s medium at 35.degree. C resulted in the intracellular multiplication of the bacteria as demonstrated by EM and immunofluorescence. In the closed experimental system used, the number of legionellae rose from 107 colony forming units (c.f.u.)/ml initially to a maximum of 1010 c.f.u./ml on day 5. Legionellae were seen in expelled phagosomes, in some amoebae filling the cytoplasm and in others in which the process of encystment appeared to have commenced. At 20.degree. C the acanthamoebae phagocytosed and digested the legionellae. The bacteria disappeared from the co-cultivation flask by day 2 but reappeared in low numbers (102 c.f.u./ml) by day 6 suggesting that even at this temperature some intra-amoebal multiplication occurred.