RopGAP4-dependent Rop GTPase rheostat control of Arabidopsis oxygen deprivation tolerance
RopGAP4-dependent Rop GTPase rheostat control of Arabidopsis oxygen deprivation tolerance
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DOI：
10.1126/science.1071505
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发表时间：
2002-06-14
期刊：
影响因子：
56.9
通讯作者：
Bailey-Serres, J
中科院分区：
文献类型：
--
作者：
Baxter-Burrell, A;Yang, ZB;Bailey-Serres, J
短暂的土壤淹水限制了根部的细胞氧气，降低了作物产量。植物对缺氧的反应包括酒精脱氢酶基因(ADH)的表达增加和乙醇发酵。Rop(Rho-like Small G Protein of Plants)鸟苷三磷酸酶(GTPase)激活蛋白4(ROPGAP4)是一种Rop失活因子，表达Rop的拟南芥基因中断后，ADH的表达增加，但对胁迫的耐受性降低。在ADH和RopGAP4表达的诱导中，依赖ROP的过氧化氢的产生是ADH和RopGAP4表达所必需的。对缺氧的耐受需要Rop激活和RopGAP4依赖的负反馈调节。这种Rop信号转导变阻器平衡了提高乙醇发酵和存活率的能力。
Transient soil flooding limits cellular oxygen to roots and reduces crop yield. Plant response to oxygen deprivation involves increased expression of the alcohol dehydrogenase gene (ADH) and ethanolic fermentation. Disruption of the Arabidopsis gene that encodes Rop (RHO-like small G protein of plants) guanosine triphosphatase (GTPase) activating protein 4 (ROPGAP4), a Rop deactivator, elevates ADH expression in response to oxygen deprivation but decreases tolerance to stress. Rop-dependent production of hydrogen peroxide via a diphenylene iodonium chloride-sensitive calcium-dependent reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is necessary for induction of both ADH and RopGAP4 expression. Tolerance to oxygen deprivation requires Rop activation and RopGAP4-dependent negative feedback regulation. This Rop signal transduction rheostat balances the ability to increase ethanolic fermentation with survival.