Blood flow through the embryonic heart outflow tract during cardiac looping in HH13-HH18 chicken embryos
Blood flow through the embryonic heart outflow tract during cardiac looping in HH13-HH18 chicken embryos
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DOI：
10.1098/rsif.2015.0652
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发表时间：
2015-10-06
影响因子：
3.9
通讯作者：
Rugonyi, Sandra
中科院分区：
文献类型：
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作者：
Midgett, Madeline;Chivukula, Venkat Keshav;Rugonyi, Sandra
血流与胚胎心脏发育有内在联系，因为血流施加的血液动力刺激机械转导机制，调节心脏生长和重塑。本研究对鸡胚HH13~HH18各阶段正常发育过程中胚胎心脏流出道(OFT)的血流变化进行了研究，以确定关键心环转变过程中血液动力学状态的变化。利用二维光相干层析成像技术同时采集结构和多普勒血流图像，提取血流速度和结构信息，估计血流动力学指标。从HH13到HH18，峰值血流速度增加了2.4倍，每搏量增加了2.1倍。管壁剪切率(WSR)和管腔直径数据表明，HH13-HH18期间血流的变化可能会在OFT中诱导剪切介导的血管扩张反应。HH13和HH18上的胚胎特有的四维计算流体动力学模型补充了实验观测，并表明OFT上的WSR分布不均匀。表征心脏循环过程中血流动力学的变化将有助于我们更好地了解正常血流影响心脏正常发育的方式。
Blood flow is inherently linked to embryonic cardiac development, as haemodynamic forces exerted by flow stimulate mechanotransduction mechanisms that modulate cardiac growth and remodelling. This study evaluated blood flow in the embryonic heart outflow tract (OFT) during normal development at each stage between HH13 and HH18 in chicken embryos, in order to characterize changes in haemodynamic conditions during critical cardiac looping transformations. Two-dimensional optical coherence tomography was used to simultaneously acquire both structural and Doppler flow images, in order to extract blood flow velocity and structural information and estimate haemodynamic measures. From HH13 to HH18, peak blood flow rate increased by 2.4-fold and stroke volume increased by 2.1-fold. Wall shear rate (WSR) and lumen diameter data suggest that changes in blood flow during HH13-HH18 may induce a shear-mediated vasodilation response in the OFT. Embryo-specific four-dimensional computational fluid dynamics modelling at HH13 and HH18 complemented experimental observations and indicated heterogeneous WSR distributions over the OFT. Characterizing changes in haemodynamics during cardiac looping will help us better understand the way normal blood flow impacts proper cardiac development.