Analyzing Single Giant Unilamellar Vesicles With a Slotline-Based RF Nanometer Sensor.
Analyzing Single Giant Unilamellar Vesicles With a Slotline-Based RF Nanometer Sensor.
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DOI：
10.1109/tmtt.2016.2536021
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发表时间：
2016-04
影响因子：
4.3
通讯作者：
Wang P
中科院分区：
文献类型：
--
作者：
Cui Y;Kenworthy AK;Edidin M;Divan R;Rosenmann D;Wang P
能够实现无试剂检测和分析单细胞的新技术对于生物和医学科学以及即时医疗服务技术的发展具有极大的意义。高灵敏度和宽带射频（RF）传感器是这种技术的有希望的候选者。在这项工作中，我们提出了一种高灵敏度和可调谐的射频传感器，它基于干涉过程并采用 100 nm 槽线结构构建。高度集中的射频场高达约 1.76×107 V/m，可实现巨型单层囊泡 (GUV) 与场之间的强烈相互作用，从而实现高灵敏度操作。我们还提供两种建模方法来从测量的散射参数中提取电池介电特性。使用 RF 传感器在 ~2 GHz、~2.5 GHz 和 ~2.8 GHz 下合成和分析不同分子组成的 GUV，初始 |S21|min 为 ~−100 dB。获得了相应的GUV介电性能。还演示了单个 GUV 的一维扫描。
Novel techniques that enable reagent free detection and analysis of single cells are of great interest for the development of biological and medical sciences as well as point-of-care health service technologies. Highly sensitive and broadband radio-frequency (RF) sensors are promising candidates for such a technique. In this work, we present a highly sensitive and tunable RF sensor, which is based on interference processes and built with a 100 nm slotline structure. The highly concentrated RF fields, up to ~1.76×107 V/m, enable strong interactions between Giant unilamellar vesicles (GUVs) and fields for high sensitivity operations. We also provide two modeling approaches to extract cell dielectric properties from measured scattering parameters. GUVs of different molecular compositions are synthesized and analyzed with the RF sensor at ~2 GHz, ~2.5 GHz, and ~2.8 GHz with an initial |S21|min of ~−100 dB. Corresponding GUV dielectric properties are obtained. A one-dimensional scanning of single GUV is also demonstrated.