Polyethylene glycol-based bidentate ligands to enhance quantum dot and gold nanoparticle stability in biological media
Polyethylene glycol-based bidentate ligands to enhance quantum dot and gold nanoparticle stability in biological media
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DOI：
10.1038/nprot.2008.243
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发表时间：
2009-01-01
期刊：
影响因子：
14.8
通讯作者：
Mattoussi, Hedi
中科院分区：
文献类型：
--
作者：
Mei, Bing C.;Susumu, Kimihiro;Mattoussi, Hedi
我们描述了一种简单而通用的方案来制备一系列基于聚乙二醇基的双齿配体，这些配体允许与胶体半导体纳米晶(量子点，QDes)和金纳米颗粒(AuNPs)发生强烈的相互作用，并促进它们在水溶液中的分散。这些配体是通过将不同链长的聚乙二醇S偶联到硫辛酸上，然后将1，2-二硫杂环戊烷部分开环合成的，从而生成了具有增强亲和力的双齿硫醇锚定基团。这些配体提供了一种直接制备量子点和AuNPs的方法，这些量子点和AuNPs对环境变化表现出更强的抵抗力，促进了它们在生物分析和活细胞成像中的有效使用。
We describe a simple and versatile scheme to prepare a series of poly(ethylene glycol)-based bidentate ligands that permit strong interactions with colloidal semiconductor nanocrystals (quantum dots, QDs) and gold nanoparticles (AuNPs) alike and promote their dispersion in aqueous solutions. These ligands are synthesized by coupling poly(ethylene glycol)s of various chain length to thioctic acid, followed by ring opening of the 1,2-dithiolane moiety to create a bidentate thiol anchoring group with enhanced affinity for CdSe-ZnS core-shell QDs. These ligands provide a straightforward means of preparing QDs and AuNPs that exhibit greater resistance to environmental changes, facilitating their effective use in bioassays and live cell imaging.