The Coiled-Coil Protein VIG1 Is Essential for Tethering Vacuoles to Mitochondria during Vacuole Inheritance of Cyanidioschyzon merolae
The Coiled-Coil Protein VIG1 Is Essential for Tethering Vacuoles to Mitochondria during Vacuole Inheritance of Cyanidioschyzon merolae
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DOI：
10.1105/tpc.109.070227
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发表时间：
2010-03-01
期刊：
影响因子：
11.6
通讯作者：
Kuroiwa, Tsuneyoshi
中科院分区：
文献类型：
--
作者：
Fujiwara, Takayuki;Kuroiwa, Haruko;Kuroiwa, Tsuneyoshi
液泡/溶酶体在内吞作用以及代谢物的储存和消化中起作用。这些细胞器由真核生物中的子代细胞遗传。然而，这种遗传的机制还不是很清楚，因为细胞含有多个随机行为的空泡。原始的红藻蓝藻有一个最少的细胞器。在这里，我们表明，分生孢子虫含有大约四个空泡，通过与分裂的线粒体结合在一起，平均分布在子细胞之间。结合是由VIG1介导的，它是一种由微阵列分析和免疫学分析鉴定的30 kD卷曲卷曲蛋白。VIG1出现在细胞质中游离空泡的表面，然后将空泡拴在线粒体上。VIG1消化后，空泡从子细胞的线粒体中释放出来。反义RNA对VIG1的抑制作用破坏了空泡的迁移。因此，VIG1在大叶锦鸡儿的液泡遗传过程中是将空泡与线粒体连接起来所必需的。
Vacuoles/lysosomes function in endocytosis and in storage and digestion of metabolites. These organelles are inherited by the daughter cells in eukaryotes. However, the mechanisms of this inheritance are poorly understood because the cells contain multiple vacuoles that behave randomly. The primitive red alga Cyanidioschyzon merolae has a minimum set of organelles. Here, we show that C. merolae contains about four vacuoles that are distributed equally between the daughter cells by binding to dividing mitochondria. Binding is mediated by VIG1, a 30-kD coiled-coil protein identified by microarray analyses and immunological assays. VIG1 appears on the surface of free vacuoles in the cytosol and then tethers the vacuoles to the mitochondria. The vacuoles are released from the mitochondrion in the daughter cells following VIG1 digestion. Suppression of VIG1 by antisense RNA disrupted the migration of vacuoles. Thus, VIG1 is essential for tethering vacuoles to mitochondria during vacuole inheritance in C. merolae.