Live imaging of mouse embryos.
Live imaging of mouse embryos.
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DOI：
10.1101/pdb.top104
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发表时间：
2011-04-01
影响因子：
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通讯作者：
Dickinson ME
中科院分区：
文献类型：
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作者：
Garcia MD;Udan RS;Hadjantonakis AK;Dickinson ME
小鼠胚胎的发育是一个动态过程，需要多种细胞类型在时空上协调迁移、增殖、凋亡、分化形成复杂结构。然而，胚胎在子宫内发育的局限性限制了我们在体内观察这些形态发生事件的能力。以前的工作使用固定样品和组织学方法，如免疫荧光或原位杂交，以解决在发育时间线内感兴趣的基因的表达或定位。然而，这种方法不允许我们跟踪胚胎发育过程中单个细胞的复杂动态运动。遗传操作方法现在允许我们标记几乎任何细胞类型或感兴趣的荧光蛋白，提供强大的见解在细胞和亚细胞分辨率的形态发生事件。体外胚胎培养方法的发展与高分辨率成像相结合，现在为观察胚胎发育过程中的形态发生事件提供了一个强大的平台。在本文中，我们讨论了活体胚胎成像在观察体内动态形态发生事件方面的优势。
The development of the mouse embryo is a dynamic process that requires the spatial and temporal coordination of multiple cell types as they migrate, proliferate, undergo apoptosis, and differentiate to form complex structures. However, the confined nature of embryos as they develop in utero limits our ability to observe these morphogenetic events in vivo. Previous work has used fixed samples and histological methods such as immunofluorescence or in situ hybridization to address expression or localization of a gene of interest within a developmental time line. However, such methods do not allow us to follow the complex, dynamic movements of individual cells as the embryo develops. Genetic manipulation methods now allow us to label virtually any cell type or protein of interest fluorescently, providing powerful insights into morphogenetic events at cellular and subcellular resolutions. The development of ex vivo embryo culture methods combined with high-resolution imaging now provides a strong platform for observing morphogenetic events as they occur within the developing embryo. In this article, we discuss the advantages of live embryo imaging for observing dynamic morphogenetic events in vivo.