Parameters for abolishing conditioned place preference for cocaine from running and environmental enrichment in male C57BL/6J mice.
Parameters for abolishing conditioned place preference for cocaine from running and environmental enrichment in male C57BL/6J mice.
复制标题
消除雄性 C57BL/6J 小鼠跑步和环境富集对可卡因的条件性位置偏好的参数。
DOI：
10.1016/j.bbr.2016.06.049
复制
发表时间：
2016
影响因子：
2.7
通讯作者：
Rhodes,JS
中科院分区：
文献类型：
--
作者：
Mustroph,ML;Pinardo,H;Merritt,JR;Rhodes,JS
有证据表明，4周的自愿跑轮运动可以消除雄性C57BL/6J小鼠对可卡因的条件位置偏好（CPP）。目的确定减少CPP所需的跑步轮暴露时间和时间，以及与不跑步的环境充实相比，跑步本身对CPP的影响程度。方法239例男性受试者连续4 d服用可卡因（10 mg/kg），每天2次，然后分为7个干预组，进行4 d的CPP检测。实验1分为两组：短时间久坐组（SS, n= 20），正常笼饲养1周；短距离跑组（SR, n= 20），用跑轮跑1周。试验2共设5组，分别设置为：短时间1周的跑步，然后是3周的久坐（SRS;n= 20）；静坐3周后跑步1周（SSR;n= 20）；长时间久坐组（LS, n= 66）在正常笼中饲养4周；长跑组（LR, n= 66）加跑轮4周；长时间环境富集组（EE, n= 27），给予玩具4周。结果各跑步组的跑步水平相近。与久坐组相比，跑步和环境丰富都降低了CPP。结论：研究结果表明，从跑步中消除可卡因CPP是强有力的，并且在干预的1周内就会发生，但可能与跑步的富集成分有关，而不是与身体活动有关。
RationaleEvidence suggests that 4 weeks of voluntary wheel running abolishes conditioned place preference (CPP) for cocaine in male C57BL/6J mice.ObjectivesTo determine the duration and timing of exposure to running wheels necessary to reduce CPP, and the extent to which the running per se influences CPP as compared to environmental enrichment without running.MethodsA total of 239 males were conditioned for 4 days twice daily with cocaine (10 mg/kg) and then split into 7 intervention groups prior to 4 days of CPP testing. Experiment 1 consisted of two groups housed as follows: short sedentary group (SS;n= 20) in normal cages for 1 week; the short running group (SR;n= 20) with running wheels for 1 week. Experiment 2 consisted of five groups housed as follows; short 1 week of running followed by a 3 week sedentary period (SRS;n= 20); a 3 week sedentary period followed by 1 week of running (SSR;n= 20); long sedentary group (LS;n= 66) in normal cages for 4 weeks; long running group (LR;n= 66) with running wheels for 4 weeks; and long environmental enrichment group (EE;n= 27) with toys for 4 weeks.ResultsLevels of running were similar in all running groups. Both running and environmental enrichment reduced CPP relative to sedentary groups.ConclusionsResults suggest that the abolishment of cocaine CPP from running is robust and occurs with as low as 1 week of intervention but may be related to enrichment component of running rather than physical activity.