A trypsin-like enzyme in acrosomal extracts of chicken, turkey and quail spermatozoa.
A trypsin-like enzyme in acrosomal extracts of chicken, turkey and quail spermatozoa.
复制标题
鸡、火鸡和鹌鹑精子顶体提取物中的一种胰蛋白酶样酶。
DOI：
10.3382/ps.0530834
复制
发表时间：
1974
期刊：
影响因子：
4.4
通讯作者：
B. Howarth
中科院分区：
文献类型：
--
作者：
B. Langford;B. Howarth
摘要对鸡、火鸡和鹌鹑精子顶体中存在的一种活性胰蛋白酶样酶进行了评价。从每个物种收集精液样本（0.5 ml），并用改良的林格溶液洗涤精子数次。制备顶体提取物，以TAME（对甲苯磺酰基- 1 -精氨酸甲酯）为底物，测定247 μ下每分钟光密度的变化，通过酯酶活性测定胰蛋白酶样酶。从鸡、火鸡和鹌鹑新近射精的精子顶体提取的酯酶活性表明，这三种物种的精子顶体中都存在一种活性的胰蛋白酶样酶。
Abstract The presence of an active trypsin-like enzyme in the acrosomes of chicken, turkey and quail spermatozoa was evaluated. Pooled semen samples (0.5 ml.) were collected from each species and the sperm washed several times with modified Ringer’s solution. Acrosomal extracts were prepared and assayed for trypsin-like enzyme by the presence of esterase activity using TAME (p-toluensulfonyl- l -arginine methyl ester) as a substrate and measuring the change in optical density per minute at 247 mμ. Esterase activity of acrosomal extracts obtained from recently ejaculated spermatozoa of the chicken, turkey and quail indicate that an active trypsin-like enzyme is present in the acrosomes of spermatozoa from all three of these species.