Capsaicin sensitivity is associated with the expression of the vanilloid (capsaicin) receptor (VR1) mRNA in adult rat sensory ganglia
Capsaicin sensitivity is associated with the expression of the vanilloid (capsaicin) receptor (VR1) mRNA in adult rat sensory ganglia
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DOI：
10.1016/s0304-3940(98)00475-3
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发表时间：
1998-07-10
影响因子：
2.5
通讯作者：
McIntyre, P
中科院分区：
文献类型：
--
作者：
Helliwell, RJA;McLatchie, LM;McIntyre, P
最近，一种香草素受体（VR1）被克隆并证明是辣椒素的靶标，辣椒素是辣椒的兴奋性毒性成分（Caterina, m.j., Schumacher, m.a., Tominaga, m.m., Rosen, t.a., Levine， J.D.和Julius， D., Nature, 389(1997) 816-824）。辣椒素的作用似乎是选择性的感觉神经元的一个子集，其中包括多模态伤害感受器。本研究描述了VRI mRNA的分布，以及辣椒素敏感性的测量，在不同胚胎起源的感觉神经节和单个交感神经节，颈上神经节（SCG）。原位杂交显示VRI mRNA在背根、三叉神经节和迷走神经节的中小神经元中表达。SCG神经元未见杂交信号。这种表达模式与全细胞电压钳测量的辣椒素敏感性相关，在类似大小的细胞中，来自背根和迷走神经节的超过80%的神经元对辣椒素敏感，但所有SCG神经元对辣椒素不敏感。(C) 1998爱思唯尔科学爱尔兰有限公司版权所有。
A vanilloid receptor (VR1) has recently been cloned and shown to be a target for capsaicin, the excitotoxic component of capsicum peppers (Caterina, M.J., Schumacher, M.A., Tominaga, M., Rosen, T.A,, Levine, J.D. and Julius, D., Nature, 389 (1997) 816-824). The effects of capsaicin appear to be selective for a subset of sensory neurones which includes polymodal nociceptors. The present study describes the distribution of VRI mRNA, together with measurements of capsaicin sensitivity, in sensory nerve ganglia of different embryological origins and a single sympathetic ganglion, the superior cervical ganglion (SCG). In situ hybridisation revealed the expression of VRI mRNA in small-to-medium-sized neurones of the dorsal root, trigeminal and vagal ganglia. No hybridisation signal was observed in the SCG neurones. This pattern of expression correlated with capsaicin sensitivity measured by whole-cell voltage clamp where, in similar sized cells, over 80% of neurones from dorsal root and vagal ganglia were capsaicin sensitive, but all SCG neurones were insensitive to capsaicin. (C) 1998 Elsevier Science Ireland Ltd. AII rights reserved.