Phosphate ester hydrolysis of biologically relevant molecules by cerium oxide nanoparticles
Phosphate ester hydrolysis of biologically relevant molecules by cerium oxide nanoparticles
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DOI：
10.1016/j.nano.2010.05.004
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发表时间：
2010-12-01
影响因子：
5.4
通讯作者：
Baker, Cheryl H.
中科院分区：
文献类型：
--
作者：
Kuchma, Melissa Hirsch;Komanski, Christopher B.;Baker, Cheryl H.
为了表征氧化铈纳米粒子 (CNP) 在生物系统中的相互作用，我们探索了 CNP 与磷酸对硝基苯酯 (pNPP)、ATP、邻磷酸-L-酪氨酸和 DNA 的磷酸酯键的反应性。 pNPP 在 pH 7 时的键断裂活性经计算为 0.860 +/- 0.010 nmol 对硝基苯酚/min/μ g CNP。有趣的是，当 CNP 与质粒 DNA 结合时，没有检测到裂解产物。虽然铈（IV）配合物通常表现出破坏磷-氧键的能力，但我们报告的反应似乎取决于铈（III）位点的可用性，而不是铈（IV）位点。我们从基本原理出发研究了去磷酸化机制，发现反应是通过类似于 S(N)2 机制的磷酸基反转进行的。 CNP 与生物相关分子的磷酸酯键相互作用的能力对其作为潜在治疗剂的用途具有重要意义。来自临床编辑：氧化铈纳米颗粒与生物相关分子的磷酸酯键相互作用的能力对其作为潜在治疗剂的用途具有重要意义。该研究小组探索了这些纳米粒子与对硝基苯磷酸盐、ATP、邻磷酸-L-酪氨酸和 DNA 的磷酸酯键的反应性。 (C) 2010 Elsevier Inc. 保留所有权利。
In an effort to characterize the interaction of cerium oxide nanoparticles (CNPs) in biological systems, we explored the reactivity of CNPs with the phosphate ester bonds of p-nitrophenylphosphate (pNPP), ATP, o-phospho-L-tyrosine, and DNA. The activity of the bond cleavage for pNPP at pH 7 is calculated to be 0.860 +/- 0.010 nmol p-nitrophenol/min/mu g CNPs. Interestingly, when CNPs bind to plasmid DNA, no cleavage products are detected. While cerium(IV) complexes generally exhibit the ability to break phosphorus-oxygen bonds, the reactions we report appear to be dependent on the availability of cerium(III) sites, not cerium(IV) sites. We investigated the dephosphorylation mechanism from the first principles and find the reaction proceeds through inversion of the phosphate group similar to an S(N)2 mechanism. The ability of CNPs to interact with phosphate ester bonds of biologically relevant molecules has important implications for their use as potential therapeutics.From the Clinical Editor: The ability of cerium oxide nanoparticles to interact with phosphate ester bonds of biologically relevant molecules has important implications for their use as potential therapeutics. This team of investigators explored the reactivity of these nanoparticles with the phosphate ester bonds of p-nitrophenylphosphate, ATP, o-phospho-L-tyrosine, and DNA. (C) 2010 Elsevier Inc. All rights reserved.