Susceptibility of Vibrio parahaemolyticus to various environmental stresses after cold shock treatment
Susceptibility of Vibrio parahaemolyticus to various environmental stresses after cold shock treatment
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DOI：
10.1016/j.ijfoodmicro.2003.10.004
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发表时间：
2004-04-15
影响因子：
5.4
通讯作者：
Chou, CC
中科院分区：
文献类型：
--
作者：
Lin, C;Yu, RC;Chou, CC
将副溶血性弧菌在20 ℃或15 ℃下冷休克处理2或4 It。冷休克对随后低温下副溶血性弧菌存活的影响（5和-18 ℃）和其他不利条件（47摄氏度，6 ppm结晶紫，1000 ppm H2 O2，25 mM醋酸和25 mM乳酸）进行了研究。无论冷休克处理如何，当储存在5或-18 ℃时，副溶血性弧菌的存活率增加，而在氯霉素存在下冷休克的细胞的存活率没有增加。在测试条件下的冷休克处理，在一般情况下，使V parahaemolyticus细胞在随后的挑战结晶紫更好地生存，而冷休克的生物体更容易受到高温（47 degreesC），H2 O2和有机酸（乳酸和乙酸）比非休克细胞。此外，冷休克处理的温度和时间影响副溶血性弧菌的冷休克反应。(C)2004 Elsevier B. V.保留所有权利。
Vibrio parahaemolyticus was subjected to cold shock treatment at 20 or 15 degreesC for 2 or 4 It. The effect of cold shock on the survival of V parahamolyticus subjected to subsequent low temperature (5 and - 18 degreesC) and other adverse conditions (47 degreesC, 6 ppm crystal violet, 1000 ppm H2O2, 25 mM acetic acid and 25 mM lactic acid) was investigated.Regardless of the cold shock treatment, survival of V parahaemolyticus increased when stored at 5 or - 18 degreesC, while no increase in survival was noted for cells cold shocked in the presence of chloramphenicol. Cold shock treatment under the conditions tested, in general, enabled V parahaemolyticus cells to survive better following subsequent challenge by crystal violet, while the cold-shocked organism was more susceptible to high temperature (47 degreesC), H2O2 and organic acids (lactic and acetic acid) than the non-shocked cells. Furthermore, the temperature and time of the cold shock treatment affected the cold shock response of V parahaemolyticus. (C) 2004 Elsevier B.V. All rights reserved.