Multiplex bead array assays for detection of soluble cytokines: Comparisons of sensitivity and quantitative values among kits from multiple manufacturers
Multiplex bead array assays for detection of soluble cytokines: Comparisons of sensitivity and quantitative values among kits from multiple manufacturers
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DOI：
10.1002/cyto.b.20021
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发表时间：
2004-09-01
影响因子：
3.4
通讯作者：
McCoy, JP
中科院分区：
文献类型：
--
作者：
Khan, SS;Smith, MS;McCoy, JP
背景：通过将多种细胞因子捕获到光谱不同的微珠上，多重微珠阵列分析允许同时对溶液中的多种细胞因子进行定量。由于几家制造商提供的试剂可以在一台仪器上对相同的细胞因子进行定量，因此需要进行比较，以确定这些试剂盒产生的数据是否相似，以及这些数据是否与酶联免疫吸附试验(ELISA)具有可比性。方法：本研究使用Luminex 100比较细胞因子检测试剂盒。使用来自LINCO Research、Bio-Rad实验室、R&D Systems和BioSource International的多路试剂盒，对7名受试者的26份血清样本进行了干扰素-γ、白细胞介素1β、6和8以及肿瘤坏死因子-a的分析。每一次检测都是按照制造商的规范进行的。通过使用每个制造商提供的参考浓度来生成标准曲线。使用研发和BioSourc的试剂盒进行IL-8的ELISA检测。结果：细胞因子水平遵循相似的模式，尽管不同试剂盒的绝对浓度不同。同一厂家生产的试剂盒中，白介素8的ELISA值和Luminex值相似。结论：由于相关的细胞因子测定在连续进行时往往很有价值，因此使用不同的试剂盒进行实验室间比较是可能的。当绝对值的比较至关重要时，应使用同一家供应商的试剂盒。供应商内部、珠粒阵列和ELISA值似乎具有可比性。2004年出版，Wiley-Liss，Inc.(匕首)
Background: Multiplex bead array assays permit simultaneous cytometric quantitation of multiple cytokines in solution by capturing these to spectrally distinct beads. Because several manufacturers offer reagents to quantitate the same cytokines on a single instrument, a comparison should be made to determine whether these kits yield similar data and whether these data are comparable to enzyme-linked immunosorbent assay (ELISA).Methods: This study compared cytokine detection kits by using Luminex 100. Twenty-six serum samples from seven subjects were analyzed for interferon-gamma, interleukins 1beta, 6, and 8, and tumor necrosis factor-a by using multiplex kits from LINCO Research, Bio-Rad Laboratories, R&D Systems, and BioSource International. Each assay was performed according to the manufacturers' specifications. Standard curves were generated by using reference concentrations supplied by each manufacturer. ELISAs for interleukin-8 were performed by using kits from R&D and BioSource.Results: Cytokine levels followed similar patterns, although absolute concentrations differed among kits. ELISA and Luminex values for interleukin-8 were similar in kits from the same manufacturer.Conclusions: Because relative cytokine measurements are often valuable when performed serially, it may be possible to make interlaboratory comparisons by using different kits. When comparison of absolute values is crucial, kits from the same supplier should be used. Within-vendor, bead array, and ELISA values appear comparable. Published 2004 Wiley-Liss, Inc.(dagger)