A panel data set on harvest and perfusion decellularization of porcine rectus abdominis.
A panel data set on harvest and perfusion decellularization of porcine rectus abdominis.
复制标题
猪腹直肌收获和灌注脱细胞化的面板数据集。
DOI：
10.1016/j.dib.2016.04.018
复制
发表时间：
2016-06
期刊：
影响因子：
1.2
通讯作者：
Badylak SF
中科院分区：
文献类型：
--
作者：
Zhang J;Cheng WY;Hu ZQ;Turner NJ;Zhang L;Wang Q;Badylak SF
在这个数据集中，我们特别描述了猪腹直肌（RA）的收获和灌注脱细胞，伴随着使用血管腐蚀铸型显示灌注脱细胞骨骼肌基质（pM-ECM）内保留的血管树。此外，还强调了成功制备pM-ECM的几个重要技术要点，包括以解剖学分离的骨骼肌为组织来源，灌注所有主要的供血和引流血管，保持内部微循环的可用性，无菌操作和无热原，经动脉或静脉顺序灌注，脱细胞后长时间冲洗。这些数据是对我们原始研究文章的补充，该文章描述了pM-ECM作为临床相关规模、三维支架与组织特异性再生血管网络模板的详细关联，“Perfusion-decellularized skeletal muscle as a three-dimensional scaffold with a vascular network template”Zhang et al.（2016）。
In this dataset, we particularly depicted the harvest and perfusion decellularization of porcine rectus abdominis (RA), accompanied with displaying of the retained vascular trees within the perfusion-decellularized skeletal muscle matrix (pM-ECM) using vascular corrosion casting. In addition, several important tips for successful pM-ECM preparation were emphasized, which including using anatomically isolated skeletal muscle as tissue source with all main feeding and draining vessels perfused, preserving the internal microcirculation availability, aseptic technique and pyrogen free in all steps, sequential perfusion via artery or vein, and longtime washing after decellularization. The data are supplemental to our original research article describing detailed associations of pM-ECM as a clinically relevant scale, three-dimensional scaffold with a vascular network template for tissue-specific regeneration, “Perfusion-decellularized skeletal muscle as a three-dimensional scaffold with a vascular network template” Zhang et al. (2016) .