T helper 1 (Th1) and Th2 characteristics start to develop during T cell priming and are associated with an immediate ability to induce immunoglobulin class switching.
T helper 1 (Th1) and Th2 characteristics start to develop during T cell priming and are associated with an immediate ability to induce immunoglobulin class switching.
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DOI：
10.1084/jem.187.8.1193
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发表时间：
1998-04-20
期刊：
影响因子：
--
通讯作者：
Acha-Orbea H
中科院分区：
文献类型：
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作者：
Toellner KM;Luther SA;Sze DM;Choy RK;Taylor DR;MacLennan IC;Acha-Orbea H
在用瑞士型小鼠乳腺肿瘤病毒[MMTV（SW）]或半抗原化蛋白初次免疫的5天内，特异性免疫球蛋白（IG）G2 a或IgG 1的各自产生提供了T辅助1（Th 1）和Th 2应答的发展的模型。抗体产生细胞产生于同源T细胞B细胞相互作用，通过在免疫后第三天分别诱导Cγ2a和Cγ1开关转录物产生来揭示。在这一天，T细胞增殖和响应于MMTV（SW）的干扰素γ和响应于半抗原化蛋白的白介素4的mRNA上调也开始。由此可见，在T细胞引发和T细胞与B细胞之间的同源相互作用的开始之间，这些应答存在最小的延迟，导致类别转换和指数生长。在T区中第一次同源T细胞与B细胞相互作用时至少部分地建立Th 1或Th 2谱。在半抗原蛋白中加入灭活的百日咳杆菌可诱导非半抗原特异性IgG 2a和IgG 1浆细胞，而抗半抗原反应仍以IgG 1为主。这表明对半抗原-蛋白的Th 2应答可以在存在大量Th 1活性的节点中进行。
The respective production of specific immunoglobulin (Ig)G2a or IgG1 within 5 d of primary immunization with Swiss type mouse mammary tumor virus [MMTV(SW)] or haptenated protein provides a model for the development of T helper 1 (Th1) and Th2 responses. The antibody-producing cells arise from cognate T cell B cell interaction, revealed by the respective induction of Cγ2a and Cγ1 switch transcript production, on the third day after immunization. T cell proliferation and upregulation of mRNA for interferon γ in response to MMTV(SW) and interleukin 4 in response to haptenated protein also starts during this day. It follows that there is minimal delay in these responses between T cell priming and the onset of cognate interaction between T and B cells leading to class switching and exponential growth. The Th1 or Th2 profile is at least partially established at the time of the first cognate T cell interaction with B cells in the T zone. The addition of killed Bordetella pertussis to the hapten–protein induces nonhapten-specific IgG2a and IgG1 plasma cells, whereas the anti-hapten response continues to be IgG1 dominated. This indicates that a Th2 response to hapten–protein can proceed in a node where there is substantial Th1 activity.