Enhanced expression of ANGPTL2 in the microvascular lesions of diabetic glomerulopathy
Enhanced expression of ANGPTL2 in the microvascular lesions of diabetic glomerulopathy
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DOI：
10.1159/000100493
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发表时间：
2007-01-01
影响因子：
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通讯作者：
Li, Lei-shi
中科院分区：
文献类型：
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作者：
Sun, Hua;Zheng, Jing-min;Li, Lei-shi
【摘要】：背景:糖尿病肾病(DN)是糖尿病最重要的微血管并发症之一。然而，其根本机制仍不清楚。我们研究了血管生成素样 2 (ANGPTL2) 的表达特征，这是我们之前的基因芯片筛选中发现的一种新型 DN 相关生长因子。方法：从 24 名 DN 患者和 8 名供体对照的肾活检中显微解剖肾小球。通过RT-PCR和免疫组化检测ANGPTL2的表达，然后与肾小球损伤的临床和病理指标相关。结果：与基因芯片实验的结果一致，与供体对照相比，ANGPTL2 在糖尿病肾小球中更频繁地表达（95% vs. 38%，chi(2) = 15.9，p < 0.01）。 ANGPTL2 mRNA 上调在微动脉瘤（22% vs. 66%，p < 0.05）、炎症流入（6 vs. 50%，p < 0.05）或内皮泡沫细胞形成（11 vs. 53%，p < 0.05）较少的肾小球中更为显着。免疫染色显示肥大的糖尿病肾小球中ANGPTL2蛋白表达上调，主要分布在足细胞中，这被认为是ANGPTL2的起源。结论：糖尿病肾小球病中ANGPTL2的上调与微血管异常和内皮炎症密切相关。 ANGPTL2可能在糖尿病肾小球病的发病机制中发挥重要作用。版权所有 (c) 2007 S. Karger AG，巴塞尔。
Background: Diabetic nephropathy (DN) is one of the most important microvascular complications of diabetes mellitus. However, the underlying mechanisms remain unclear. We studied the expression characteristics of angiopoietin-like 2 (ANGPTL2), a novel DN-associated growth factor identified in our previous gene chip screening. Methods: Glomeruli were microdissected from renal biopsies from 24 patients with DN and 8 donor controls. The expression of ANGPTL2 was assessed by RT-PCR and immunohistochemistry, and then correlated with clinical and pathological indices of glomerular injury. Results: Consistent with the results of the gene chip experiment, abundant expression of ANGPTL2 was found more frequently in diabetic glomeruli as compared to donor controls (95 vs. 38%, chi(2) = 15.9, p < 0.01). ANGPTL2 mRNA upregulation was more prominent in glomeruli with less microaneurysm (22 vs. 66%, p < 0.05), inflammatory influx (6 vs. 50%, p < 0.05) or endothelial foam cell formation (11 vs. 53%, p < 0.05). Immunostaining revealed an upregulation of ANGPTL2 protein in hypertrophied diabetic glomeruli, mainly distributed in podocytes, which were supposed to be the origin of ANGPTL2. Conclusion: The upregulation of ANGPTL2 in diabetic glomerulopathy shows a close relationship to abnormal microvasculature and endothelial inflammation. ANGPTL2 may play an important role in the pathogenesis of diabetic glomerulopathy. Copyright (c) 2007 S. Karger AG, Basel.