Simple quality assessment approach for herbal extracts using high performance liquid chromatography-UV based metabolomics platform
Simple quality assessment approach for herbal extracts using high performance liquid chromatography-UV based metabolomics platform
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DOI：
10.1016/j.chroma.2009.12.031
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发表时间：
2010-02-19
影响因子：
4.1
通讯作者：
Xu, Liang
中科院分区：
文献类型：
--
作者：
Lan, Ke;Zhang, Yan;Xu, Liang
缺乏适当或公认的研究方法一直是研究草药的主要障碍。在本研究中，没有使用普遍的联用色谱法，而是使用配备紫外线检测器的普通高效液相色谱法（HPLC-UV）和多变量统计分析来评估沙棘总黄酮（TFS）（沙棘浆果的 85% 乙醇提取物）的质量。开发、验证和组合两种互补的 HPLC-UV 方法，综合确定 TFS 中的成分。组合分析数据的主成分分析 (PCA) 和偏最小二乘判别分析 (PLS-DA) 表明，六批 TFS 可以很好地区分。使用 PLS-DA 负载矩阵的 Ward 最小方差法的层次聚类分析 (HCA) 证明了已知成分（槲皮素、山奈酚、异鼠李素、齐墩果素）。 TFS 中的三种未知成分对质量差异有显着贡献 PLS 回归模型表明，本方法的结果与总黄酮的含量有很好的相关性，而总黄酮现在是 ITS 的质量控制方法。这项研究的结果表明，所提出的方法对于一些广泛使用的草药提取物的二元性重新评估是可靠的。 Crown 版权所有 (C) 2009 由 Elsevier B.V. 出版。保留所有权利
A lack of adequate or accepted research methodology has been a major obstacle to study herbal medicines In this study, instead of the prevalent hyphenated chromatographies, common high performance liquid chromatography equipped with ultraviolet detector (HPLC-UV) and multivariate statistical analysis were utilized to assess the qualities of total flavones of sea buckthorn (TFS), an 85% ethanol extract of the sea buckthorn berries. Two complementary HPLC-UV methods were developed, validated and combined to comprehensively determine the ingredients in TFS Principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) of the combined analytical data showed that the six batches of TFS could be well differentiated Hierarchical cluster analysis (HCA) using Ward's minimum variance method of the PLS-DA loading matrix demonstrated the known ingredients (quercetin, kaempferol, isorhamnetin, oleanolic acid and ursolic acid) and three unknown ingredients in TFS significantly contributed to the quality differences A PLS regression model indicated that the results of the present method correlated well with the content of total flavones, which is now the quality control approach of ITS Results from this study indicated that the proposed method is reliable for the duality reassessment of some widely used herbal extracts Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved