Quantitative analysis of copy number variants based on real-time LightCycler PCR.

Quantitative analysis of copy number variants based on real-time LightCycler PCR.
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DOI:
10.1002/0471142905.hg0721s80
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发表时间:
2014-01-21
影响因子:
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通讯作者:
Chung, Wendy K
Chung, Wendy K
中科院分区:
其他
文献类型:
--
作者:
Ma, Lijiang;Chung, Wendy K

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定量实时PCR是通过使用荧光或嵌入染料以真实的时间可视化的PCR,所述荧光或嵌入染料用于测量基因表达或基因定量,包括连续的基因缺失或重复。描述了一种简单的方法来定量人类样本中的DNA拷贝数。
Quantitative real-time PCR is PCR visualized in real time by the use of fluorescent or intercalating dyes used to measure gene expression or gene quantification including including contiguous gene deletions or duplications. A simple method is described to quantify DNA copy number from human samples.