Somatic cell fusion in the study of glucocorticoid action.
Somatic cell fusion in the study of glucocorticoid action.
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糖皮质激素作用研究中的体细胞融合。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
E. Thompson
中科院分区：
文献类型：
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作者：
U. Gehring;E. Thompson
简要介绍了细胞融合和杂交细胞形成的基本现象，并讨论了体细胞杂交在分化细胞功能表达研究中的潜力。细胞杂交技术已应用于两种类型的细胞对糖皮质激素的反应。在可诱导细胞与不可诱导细胞的杂交中，研究了特异性蛋白的诱导作用。大多数研究涉及肝脏特异性酶酪氨酸转氨酶，在大多数肝癌和非肝细胞之间的杂交中，酪氨酸转氨酶的诱导性被消除。然而，在染色体分离后，这些杂交细胞中的一些重新出现了诱导性。讨论了目前关于诱导性的细胞控制的观点。细胞杂交研究中研究的另一个主要的糖皮质激素应答系统由淋巴样细胞组成，这些细胞在暴露于类固醇时被杀死。这些敏感细胞与几种糖皮质激素抗性淋巴细胞系杂交，发现敏感性优于抗性。然而，与野生型亲本细胞相比，敏感淋巴样细胞和抗性淋巴样细胞之间的杂交显示出抗性发生的频率增加。在糖皮质激素特异性受体系统存在缺陷的不同类型的耐药细胞之间的杂交中，没有发现类固醇敏感性的互补。
The basic phenomena of cell fusion and hybrid cell formation are briefly described and the potential of somatic cell hybridization in studies on the expression of differentiated cellular functions is discussed. The technique of cell hybridization has been applied to two types of cellular responses to glucocorticoids. The induction of specific proteins has been investigated in hybrids of inducible cells with uninducible cells. Most studies dealt with the liver-specific enzyme tyrosine aminotransferase, whose inducibility was extinguished in the majority of the hybrids between hepatoma and nonliver cells. However, upon chromosome segregation, inducibility reappeared in some of these hybrid cells. The current ideas about cellular control of inducibility are discussed. The other major glucocorticoid-responsive system investigated in cell hybridization studies consists of lymphoid cells which are killed when exposed to the steroid. Such sensitive cells were hybridized with several types of glucocorticoid-resistant lymphoid lines, and sensitivity was found to be dominant over resistence. Hybrids between sensitive and resistant lymphoid cells, however, showed an increase in the frequency at which resistance occurred as compared to the rate observed with the wild-type parental cells. No complementation to steroid sensitivity was found in hybrids between different types of resistant cells with defects in the glucocorticoid-specific receptor system.