Denitrification using PBS as carbon source and biofilm support in a packed-bed bioreactor
Denitrification using PBS as carbon source and biofilm support in a packed-bed bioreactor
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DOI：
10.1007/s11356-012-0926-9
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发表时间：
2012
影响因子：
5.8
通讯作者：
Weizhong Wu;Lu-Hua Yang;Jianlong Wang
中科院分区：
文献类型：
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作者：
Weizhong Wu;Lu-Hua Yang;Jianlong Wang
可生物降解聚合物作为碳源和生物膜载体用于去除水中硝酸盐是一种很有吸引力的生物脱氮方法。研究了以可生物降解聚合物聚丁二酸丁二醇酯（PBS）为碳源和生物膜载体的填充床生物反应器的脱氮性能和微生物群落结构。用紫外分光光度计测定NO3-N浓度。NO2-N浓度采用盐酸萘乙二胺分光光度法测定。使用TOC分析仪测量总有机碳（TOC）。使用环境扫描电子显微镜（ESEM）观察样品的形态。采用焦磷酸测序法对微生物群落进行分析。试验结果表明，硝酸盐的平均去除率为95%。环境扫描电镜观察和红外光谱分析表明微生物利用前后PBS颗粒的变化。焦磷酸测序结果表明，β-变形菌占优势，大多数PBS降解菌归属于丛毛单胞菌科。反硝化细菌占总菌群的13.02%. PBS颗粒是微生物生长的适宜载体和碳源。
Biodegradable polymer was used as carbon source and biofilm support for nitrate removal from aqueous solution as an attractive alternative for biological denitrification. The objective of this paper was to investigate the denitrification performance and microbial community of a packed-bed bioreactor using poly (butanediol succinate) (PBS), a biodegradable polymer, as carbon source and biofilm support. NO3–N concentration was determined by UV spectrophotometer. NO2–N concentration was assayed by hydrochloric acid naphthyl ethylenediamine spectrophotometry method. Total organic carbon (TOC) was measured using a TOC analyzer. The morphology of the samples was observed using an environmental scanning electron microscope (ESEM). The microbial community was analyzed by pyrosequencing method. The experimental results showed that an average removal efficiency of nitrate was 95 %. ESEM observation and FTIR analysis indicated the changes of PBS granules before and after microbial utilization. Pyrosequencing results showed that Betaproteobacteria predominated, and most of PBS-degrading denitrifying bacteria were assigned to the family Comamonadaceae. Denitrifying bacteria accounted for 13.02 % in total population. The PBS granules were suitable support and carbon source for denitrifying bacteria.