RNA-Seq profiling of circular RNA in human lung adenocarcinoma and squamous cell carcinoma
RNA-Seq profiling of circular RNA in human lung adenocarcinoma and squamous cell carcinoma
复制标题
人肺腺癌和鳞状细胞癌中环状 RNA 的 RNA-Seq 分析
DOI：
10.1186/s12943-019-1061-8
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发表时间：
2019-09-04
期刊：
影响因子：
37.3
通讯作者：
Li, Weimin
中科院分区：
文献类型：
--
作者：
Wang, Chengdi;Tan, Shuangyan;Li, Weimin
新出现的证据表明，环状RNA（circRNAs）在肿瘤中异常表达，并且可作为癌症的预后标志物。然而，circRNAs在非小细胞肺癌（NSCLC）中的表达模式和临床意义仍然不清楚。在本研究中，我们在对10对肺腺癌（LUAD）和肺鳞状细胞癌（LUSC）进行核糖体RNA去除和核糖核酸酶R消化以富集circRNAs后，分析了circRNA的表达情况。结合五种circRNA计算程序，我们发现LUAD和LUSC不仅具有共同的表达模式，还呈现出不同的circRNA表达特征。此外，受试者工作特征（ROC）曲线分析表明，hsa_circ_0077837和hsa_circ_0001821可作为LUAD和LUSC两者的潜在生物标志物，而hsa_circ_0001073和hsa_circ_0001495可分别作为LUAD和LUSC的诊断/分型标志物。因此，我们的研究结果强调了circRNAs在NSCLC中的重要诊断潜力。
Emerging evidences demonstrate that circular RNAs (circRNAs) are abnormally expressed in tumors and could serve as prognostic markers for cancers. However, the expression patterns and clinical implications of circRNAs in non-small cell lung cancer (NSCLC) remain obscure. In this study, we profiled circRNA expressions in 10 pairs of lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC) after ribosomal RNA-depletion and RNase R digestion to enrich circRNAs. Combining five circRNA computational programs, we found that LUAD and LUSC not only share common expression patterns, but also exhibit distinct circRNA expression signatures. Moreover, the Receiver Operating Characteristic (ROC) curve analysis indicated that hsa_circ_0077837 and hsa_circ_0001821 could serve as potential biomarkers for both LUAD and LUSC, while hsa_circ_0001073 and hsa_circ_0001495 could be diagnostic/subtyping marker for LUAD and LUSC, respectively. Therefore, our findings highlight the important diagnostic potential of circRNAs in NSCLC.