Role of staphylococcal phage and SaPI integrase in intra- and interspecies SaPI transfer
Role of staphylococcal phage and SaPI integrase in intra- and interspecies SaPI transfer
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DOI：
10.1128/jb.00619-07
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发表时间：
2007-08-01
影响因子：
3.2
通讯作者：
Penades, Jose R.
中科院分区：
文献类型：
--
作者：
Maiques, Elisa;Ubeda, Carles;Penades, Jose R.
SaPIbov 2是葡萄球菌致病岛SaPI家族的成员，与SaPIbov 1非常密切相关。通常，某些温和的病毒可以诱导一个或多个这些岛的切除和复制，并可以将它们包装成与其基因组大小相称的特殊小噬菌体样颗粒（称为切除-复制-包装[ERP]循环）。我们已经使用SaPIbov 2在一定程度上深入研究了噬菌体-SaPI相互作用，特别是其整合酶的作用。我们在这里证明，SaPIbov 2可以被不同的葡萄球菌感染诱导复制。复制后，SaPIbov 2被有效地包被并转移至受体生物体，包括不同的非金黄色葡萄球菌，其中其通过自身编码的整合酶（Int）在SaPI特异性附着位点att（C）处整合。不能诱导SaPIbov 2 ERP周期的噬菌体可以通过recA依赖性经典广义转导转移岛，也可以通过需要在受体中而不是在供体中表达SaPIbov 2 int的新机制转移岛。有人建议，这一机制涉及的标准转导片段，含有完整的岛，然后由int介导的切除，环化，并在受体中的整合。
SaPIbov2 is a member of the SaPI family of staphylococcal pathogenicity islands and is very closely related to SaPIbov1. Typically, certain temperate phages can induce excision and replication of one or more of these islands and can package them into special small phage-like particles commensurate with their genome sizes (referred to as the excision-replication-packaging [ERP] cycle). We have studied the phage-SaPI interaction in some depth using SaPIbov2, with special reference to the role of its integrase. We demonstrate here that SaPIbov2 can be induced to replicate by different staphylococcal phages. After replication, SaPIbov2 is efficiently encapsidated and transferred to recipient organisms, including different non-Staphylococcus aureus staphylococci, where it integrates at a SaPI-specific attachment site, att(C), by means of a self-coded integrase (Int). Phages that cannot induce the SaPIbov2 ERP cycle can transfer the island by recA-dependent classical generalized transduction and can also transfer it by a novel mechanism that requires the expression of SaPIbov2 int in the recipient but not in the donor. It is suggested that this mechanism involves the encapsidation of standard transducing fragments containing the intact island followed by int-mediated excision, circularization, and integration in the recipient.