Development of paternally‐inherited Y chromosome simple sequence repeats of sika deer and their application in genetic structure, artificial introduction, and interspecific hybridization analyses
Development of paternally‐inherited Y chromosome simple sequence repeats of sika deer and their application in genetic structure, artificial introduction, and interspecific hybridization analyses
复制标题
DOI：
10.1002/1438-390x.12109
复制
发表时间：
2022-01
影响因子：
1.7
通讯作者：
Toshihito Takagi;Y. Tsuda;H. Torii;H. Tamate;S. Kaneko;J. Nagata
中科院分区：
文献类型：
--
作者：
Toshihito Takagi;Y. Tsuda;H. Torii;H. Tamate;S. Kaneko;J. Nagata
哺乳动物生态学和进化中性别偏向传播的影响可以通过关注母系或父系遗传的 DNA 多态性来阐明。在梅花鹿中，基于线粒体DNA变异的研究已经阐明了母系谱系的遗传结构。然而，由于Y染色体序列点突变数量有限，父系谱系的遗传结构尚未得到很好的分析。在这项研究中，我们重点关注 Y 染色体中高度多态性简单重复序列 (SSR) 的突变，并开发了 16 个 Y 染色体 SSR 标记来评估梅花鹿的雄性偏向扩散。从这 16 个位点总共检测到 55 个等位基因和 31 个多位点单倍型，揭示了人群之间明显的遗传分化 (F′ST=0.783)。特别是种子岛和屋久岛的本地个体具有独特的等位基因，并且从台湾引入了外来个体。这些标记不仅对于评估历史上男性介导的扩散、遗传结构和日本本土种群的人口统计非常有用，而且对于评估人工引入对杂交的影响，特别是等位基因从逃逸养殖个体向本土种群的渗入也非常有用。
The effect of sex‐biased dispersal in mammalian ecology and evolution can be elucidated by focusing on maternally or paternally inherited DNA polymorphisms. In sika deer, the genetic structure of the maternal lineage has been clarified by studies based on mitochondrial DNA variation. However, the genetic structure of the paternal lineage has not been well analyzed due to the limited number of point mutations in Y chromosome sequences. In this study, we focused on mutations of highly polymorphic simple sequence repeats (SSRs) in the Y chromosome and developed 16 Y chromosome SSR markers to evaluate male‐biased dispersal in sika deer. In total, 55 alleles and 31 multi‐locus haplotypes were detected from these 16 loci, revealing clear genetic differentiation among populations (F′ST= 0.783). In particular, there were unique alleles for the native individuals on Tanegashima and Yakushima Islands and introduced exotic individuals from Taiwan. These markers are highly useful for evaluating not only historical male‐mediated dispersal, genetic structure, and demography of the native populations in Japan, but also the impact of artificial introductions on hybridization, especially the introgression of alleles from escaped farmed individuals to native populations.