Maternal fructose intake disturbs ovarian estradiol synthesis in rats
Maternal fructose intake disturbs ovarian estradiol synthesis in rats
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DOI：
10.1016/j.lfs.2018.04.006
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发表时间：
2018-06-01
期刊：
影响因子：
6.1
通讯作者：
Ohashi, Koji
中科院分区：
文献类型：
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作者：
Munetsuna, Eiji;Yamada, Hiroya;Ohashi, Koji
目的：最近果糖摄入量的增加引起了对潜在不良代际效应的关注，因为母体果糖摄入可能导致后代的生理功能障碍。然而，目前还没有关于母体过量果糖对生殖组织如卵巢的影响的报道。值得注意的是，母体宫内环境已被证明会影响后代的卵巢发育。由于果糖被转移到胎儿体内，过量的果糖摄入可能会影响后代卵巢的发育。由于卵巢的发育及其功能是由17 - β -雌二醇维持的，因此我们研究了过量的母体果糖摄入是否会影响子代卵巢雌二醇的合成。大鼠在妊娠期和哺乳期服用20%的果糖溶液。断奶后，分离子代卵巢。主要发现：果糖喂养的幼鼠的后代显示StAR和P450(17 α) mRNA水平降低，蛋白表达水平降低。相反，在没有mRNA表达改变的情况下，发现P450arom蛋白水平降低。与这些现象一致的是，观察到循环中的雌二醇水平下降。此外，雌激素受体a （ER α）蛋白水平也下调。与此同时，ER α的转录靶点孕酮受体mRNA表达减少。这些结果表明，母体果糖可能会改变后代的卵巢生理。
Aims: Recent increases in fructose consumption have raised concerns regarding the potential adverse inter-generational effects, as maternal fructose intake may induce physiological dysfunction in offspring. However, no reports are available regarding the effect of excess maternal fructose on reproductive tissues such as the ovary. Notably, the maternal intrauterine environment has been demonstrated to affect ovarian development in the subsequent generation. Given the fructose is transferred to the fetus, excess fructose consumption may affect offspring ovarian development. As ovarian development and its function is maintained by 17 beta-estradiol, we therefore investigated whether excess maternal fructose intake influences offspring ovarian estradiol synthesis. Rats received a 20% fructose solution during gestation and lactation. After weaning, offspring ovaries were isolated.Key findings: Offspring from fructose-fed dams showed reduced StAR and P450(17 alpha) mRNA levels, along with decreased protein expression levels. Conversely, attenuated P450arom protein level was found in the absence of mRNA expression alteration. Consistent with these phenomena, decreased circulating levels of estradiol were observed. Furthermore, estrogen receptor a (ER alpha) protein levels were also down-regulated. In accordance, the mRNA for progesterone receptor, a transcriptional target of ER alpha, was decreased. These results suggest that maternal fructose might alter ovarian physiology in the subsequent generation.