CONVENTIONAL AND ENHANCED PLAQUE NEUTRALIZATION ASSAY FOR POLIO ANTIBODY
CONVENTIONAL AND ENHANCED PLAQUE NEUTRALIZATION ASSAY FOR POLIO ANTIBODY
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DOI：
10.1016/0166-0934(83)90046-0
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
ALBRECHT, P
中科院分区：
文献类型：
--
作者：
BOONE, EJ;ALBRECHT, P
人类喉癌HEp-2细胞比非洲绿猴肾Vero细胞更适合作为细胞底物用于脊髓灰质炎病毒野生株或减毒株的斑块测定。用空斑中和法测定脊髓灰质炎抗体的灵敏度比用病毒细胞病变效应法测定抗体的灵敏度平均高3.4-4.8倍。通过延长血清-病毒相互作用的时间，对病毒中和的效果最为显著。将病毒-抗血清混合物在36度下孵育1小时改为20小时。C提高了对所有3种脊髓灰质炎病毒类型的抗体滴度。11到28倍。异源抗球蛋白增强脊髓灰质炎病毒中和作用的效果明显低于其他病毒抗体系统。所描述的病毒空斑中和技术应能够在特殊情况下测量微量抗体。
Human laryngeal carcinoma HEp-2 cells were more suitable as a cell substrate than African green monkey kidney Vero cells for plaque assay of wild or attenuated strains of poliovirus. Polio antibody titration by plaque neutralization was on the average 3.4-4.8 times more sensitive than antibody titration by virus cytopathic effect assay. The most pronounced effect on virus neutralization was achieved by extending the time of serum-virus interaction. Incubating the virus-antiserum mixture for 20 h instead of 1 h at 36.degree. C increased antibody titer to all 3 poliovirus types .apprx. 11- to 28-fold. Potentiation of poliovirus neutralization by heterologous antiglobulin was considerably less effective than with other virus-antibody systems. The virus plaque neutralization technique described should be capable of measuring minute amounts of antibody as required in special circumstances.