The Na(+)/Ca(2+) exchanger NCKX4 governs termination and adaptation of the mammalian olfactory response.
The Na(+)/Ca(2+) exchanger NCKX4 governs termination and adaptation of the mammalian olfactory response.
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DOI：
10.1038/nn.2943
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发表时间：
2011-11-06
影响因子：
25
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中科院分区：
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感觉知觉需要感觉感受器细胞对刺激信息进行准确编码。在这里，我们发现NCKX4是一种钾依赖的钠/钙交换分子，对于脊椎动物嗅觉神经元(OSN)的快速反应终止和适当适应是必需的。NCKX4−/−小鼠OSN表现出显著延长的反应和更强的适应能力。单细胞电生理分析表明，与感觉转导相关的OSNs中的大部分Na+依赖的钙离子交换是由NCKX4引起的，NCKX4−/−小鼠OSNs在重复刺激下编码动作电位的能力不足。嗅觉特异的Nockx4基因敲除小鼠定位气味来源的能力降低，体重减轻。这些结果证实了NCKX4在形成嗅觉反应中的作用，并表明快速反应终止和外周感觉感受器细胞的适当适应调节感觉系统以获得最佳知觉。
Sensory perception requires accurate encoding of stimulus information by sensory receptor cells. Here, we identify NCKX4, a potassium – dependent Na+/Ca2+ exchanger, to be necessary for rapid response termination and proper adaptation of vertebrate olfactory sensory neurons (OSNs). Nckx4−/− mouse OSNs display substantially prolonged responses and stronger adaptation. Single – cell electrophysiological analyses demonstrate that the majority of Na+ – dependent Ca2+ exchange in OSNs relevant to sensory transduction is due to NCKX4 and that Nckx4−/− mouse OSNs are deficient in encoding action potentials upon repeated stimulation. Olfactory – specific Nckx4 knockout mice have a reduced ability to locate an odorous source and lower body weights. These results establish the role of NCKX4 in shaping olfactory responses and suggest that rapid response termination and proper adaptation of peripheral sensory receptor cells tune the sensory system for optimal perception.