Transcriptome analysis reveals roles of polian vesicle in sea cucumber Apostichopus japonicus response to Vibrio splendidus infection
Transcriptome analysis reveals roles of polian vesicle in sea cucumber Apostichopus japonicus response to Vibrio splendidus infection
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DOI：
10.1016/j.cbd.2021.100877
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发表时间：
2021-07-12
影响因子：
3
通讯作者：
Li, Qiang
中科院分区：
文献类型：
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作者：
Guo, Liyuan;Wang, Zhenhui;Li, Qiang
通过细胞耗竭和异种细胞注射两种方式，海参原被认为是一种造血性和炎性反应器官。在本研究中，为了揭示刺参囊泡对病原菌的免疫应答作用及其免疫机制，我们首先利用RNA高通量测序技术对刺参攻击刺参的刺参囊壁细胞进行了转录组学比较分析。简单地说，在总共清理了低质量的读数之后，获得了465,356,848个干净读数。大约73%的测序读数可以与日本按蚊的参考基因组比对。CG(对照组)与TG(感染后24小时组)、CG与TG(感染后72小时组)、TG(感染后24小时与TG 72小时组)的DEG值分别为3762、1391和3258。基因本体论(GO)注释分析表明，与细胞过程、细胞、结合和催化活性等过程相关的基因在感染华支睾吸虫后均显著诱导。KEGG富集率分析表明，与对照组相比，TG组在Toll样受体(TLR)信号通路、补体和凝血级联、抗原处理和递呈以及IL-17信号通路等方面均有丰富，而与细胞增殖相关的核糖体生物发生、DNA复制和细胞周期等途径则主要在72h组较CG组富集。此外，还选择了6个重要的DEG，其表达谱与定量聚合酶链式反应的结果一致。总体而言，我们对现有数据的分析表明，Polian囊泡可能在日本血吸虫免疫反应的调节中发挥重要作用，并为了解Polian囊泡在应对病原体感染时的造血功能提供了新的见解。
Polian vesicle is originally regarded as a hematopoietic and inflammatory response organ in sea cucumber by the operations of cell depletion and heterogeneous cells injection, respectively. In the present study, to reveal the role and immune mechanisms of polian vesicle in response to pathogen, Vibrio splendidus, we first performed a comparative transcriptome analysis for the cells from polian vesicle wall in V. splendidus-challenged Apostichopus japonicus through RNA high-throughput sequencing technology. Briefly, 465,356,848 clean reads were obtained after cleaning up low-quality reads in total. Approximately 73% of the sequenced reads could be aligned to the reference genome of A. japonicus. The DEGs of CG (control group) vs TG 24 h (24 h post-infection group), CG vs TG 72 h (72 h post-infection group) and TG 24 h vs TG 72 h were 3762, 1391 and 3258, respectively. Gene Ontology (GO) annotation assay revealed that those genes associated with the processes such as cell process, cell, binding and catalytic activity were significantly induced in all three groups post V. splendidus infection. KEGG enrichment analysis suggested the DEGs in TG 24 h were enriched in Toll-like receptor (TLR) signaling pathway, complement and coagulation cascades, antigen processing and presentation and IL-17 signaling pathway compared with that in CG, while the pathways including ribosome biogenesis in eukaryotes, DNA replication, and cell cycle related with cell proliferation were mainly enriched in TG 72 h than that of CG. Furthermore, six important DEGs were chosen and showed the consistent expression patterns with the results of RNA-seq by qPCR. Overall, our analysis towards the current data demonstrates that polian vesicle may play an essential role in the regulation of immune response in A. japonicus and provide new insights into hematopoietic function of polian vesicle in response to pathogen infection.