A SURVEY OF TRYPANOSOME INFECTIONS IN WILD UNGULATES IN LUANGWA VALLEY, ZAMBIA
A SURVEY OF TRYPANOSOME INFECTIONS IN WILD UNGULATES IN LUANGWA VALLEY, ZAMBIA
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DOI：
10.1080/00034983.1969.11686618
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发表时间：
1969-01-01
影响因子：
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通讯作者：
KEYMER, IF
中科院分区：
文献类型：
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作者：
KEYMER, IF
材料和方法用于分离锥虫的方法与Kinghorn等所用的方法相似。（一九一三年）。用枪伤或死亡时的心脏血液制成薄血膜，用甲醇固定，用吉姆萨染色剂染色，并检查至少2小时。将21只动物的新鲜肝素化血液腹腔内接种到实验室大鼠体内，通常立即接种，并且总是在死亡后4S分钟内接种。将大鼠饲养在防采采蝇（Glossina spp.）的容器中。还维持未接种的对照，所有对照均保持未感染。每隔不超过4天检查所有大鼠血液中的锥虫。
MATERIALS AND METHODSThe methods used for the isolation of trypanosomes were similar to those used by Kinghorn et al.(1913). Thin blood films were made from shot-wounds or heart blood at the time of death; they were fixed with methyl alcohol, stained with Giemsa's stain, and examined for at least two hours.Fresh heparinized blood from 21 animals was inoculated intraperitoneally into laboratory rats, usually immediately and always within 4S minutes of death. The rats were kept in containers protected from tsetse flies (Glossina spp.). Uninoculated controls, all of which remained uninfected, were also maintained. The blood of all rats was examined for trypanosomes at intervals not exceeding four days.