Creation of a female rabbit model for intrauterine adhesions using mechanical and infectious injury
Creation of a female rabbit model for intrauterine adhesions using mechanical and infectious injury
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利用机械损伤和感染损伤建立雌性兔宫腔粘连模型
DOI：
10.1016/j.jss.2012.11.009
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发表时间：
2013-07-01
影响因子：
2.2
通讯作者：
He, Yuan-Li
中科院分区：
文献类型：
--
作者：
Liu, Fang;Zhu, Zhi-Jun;He, Yuan-Li
背景：宫腔粘连（IUA）与继发性闭经、不孕和复发性流产有关。 IUA动物模型将有助于研究IUA的发病机制和病理变化以及探索新的治疗方法。 材料与方法：将80只雌性家兔随机分为5组：机械损伤组（16只）、感染性损伤组（16只）、双重损伤组（16只）、实验对照组（16只）和正常组（16只）。采用清宫、宫腔留置、脂多糖手术缝合48 h、清宫后保留缝线48 h 3种方法建立模型。实验对照组将无菌手术缝线留在子宫腔内48小时。术后0、24、48、72 h和7、14、28 d监测组织学变化。结果：实验表明，单纯刮宫或感染损伤的子宫内膜是可以修复的。虽然双重损伤使子宫内膜再生严重受损，但子宫内膜间质纤维化面积占子宫内膜总面积的比例显着增加（P < 0.01），子宫内膜腺体数量显着减少（P < 0.01）。结论：双重损伤方法可以建立稳定的兔IUA模型。 (C) 2013 Elsevier Inc. 保留所有权利。
Background: Intrauterine adhesions (IUA) are associated with secondary amenorrhea, infertility, and recurrent pregnancy loss. An IUA animal model would contribute to research on the pathogenesis and pathologic changes of IUA and the exploration of new treatments.Materials and methods: Eighty female rabbits were randomly divided into five groups: mechanical injury (16), infectious injury (16), dual injury (16), experimental control (16), and normal (16). Three methods were applied to establish the model: uterine curettage, uterine cavity left alone, lipopolysaccharide surgical suture in place for 48 h, and suture retention for 48 h after curettage. A sterile surgical suture was left in the uterine cavity for 48 h in the experimental control group. Histologic changes were monitored at 0, 24, 48, and 72 h and 7, 14, and 28 d after operation.Results: The experiments revealed that endometrium injured by simple curettage or infection could be repaired. Although endometrial regeneration was severely impaired by dual injury, the ratio of the area with endometrial stromal fibrosis to total endometrial area significantly increased (P < 0.01), and the number of endometrial glands was significantly reduced (P < 0.01).Conclusions: The method of dual injury can establish a stable rabbit IUA model. (C) 2013 Elsevier Inc. All rights reserved.