Pulmonary delivery of respiratory syncytial virus DNA vaccines using macroaggregated albumin particles
Pulmonary delivery of respiratory syncytial virus DNA vaccines using macroaggregated albumin particles
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DOI：
10.1016/j.vaccine.2003.11.050
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发表时间：
2004-06-02
期刊：
影响因子：
5.5
通讯作者：
Tripp, RA
中科院分区：
文献类型：
--
作者：
Harcourt, JL;Anderson, LJ;Tripp, RA
At present there is no safe and effective vaccine for respiratory syncytial virus (RSV). DNA vaccines encoding RSV surface glycoproteins are one option being examined. Current methods to deliver DNA vaccines generally require repeated high dose intramuscular or intradermal administration for effectiveness. In this study, we examine the efficacy of pulmonary DNA vaccination using low dose DNA vaccines encoding the RSV F glycoprotein conjugated to macroaggregated albumin (MAA-F). Single vaccination of BALB/c mice with 1 mug MAA-F was ineffective, however mice boosted with an additional 1 mug MAA-F, or vaccinated a single time with 10 mug MAA-F, developed substantially improved immunity associated with reduced viral titers, increased anti-F antibody responses, and enhanced Th1 and Th2 intracellular cytokine responses. This Study shows that MAA may be a useful carrier for RSV DNA vaccines. (C) 2003 Elsevier Ltd. All rights reserved.
