Chitosan-iron oxide nanocomposite based electrochemical aptasensor for determination of malathion
Chitosan-iron oxide nanocomposite based electrochemical aptasensor for determination of malathion
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DOI：
10.1016/j.aca.2016.08.015
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发表时间：
2016-10-05
影响因子：
6.2
通讯作者：
Kaur, Navpreet
中科院分区：
文献类型：
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作者：
Prabhakar, Nirmal;Thakur, Himkusha;Kaur, Navpreet
以壳聚糖-氧化铁纳米复合物（CHIT-IO）为载体，在氟锡氧化物（FTO）上制备了一种用于马拉硫磷检测的电化学适体传感器。采用共沉淀法制备了氧化铁纳米粒子，并通过透射电镜和紫外-可见光谱对其进行了表征。以链霉亲和素为连接分子，将马拉硫磷特异性的生物素化DNA适配体序列固定在氧化铁掺杂的壳聚糖/FTO电极上。进行了各种表征研究，如场发射扫描电子显微镜（FE-SEM），傅里叶变换红外光谱（FT-IR）和电化学研究，以证明生物电极的成功制造。优化了适体浓度、响应时间、电极稳定性和重复使用性等实验参数。适体固定化壳聚糖-氧化铁纳米复合材料（APT/SAICHIT-IO/FTO）生物电极在15 min内表现出约0.001 ng/mL的LOD，并且加标研究显示从莴苣叶和土壤样品中约80-92%的马拉硫磷回收率。(C)2016爱思唯尔B. V.保留所有权利。
An electrochemical aptasensor based on chitosan-iron oxide nanocomposite (CHIT-IO) film deposited on fluorine tin Oxide (FTO) was developed for the detection of malathion. Iron oxide nanoparticles were prepared by co-precipitation method and characterized by Transmission electron microscopy and UV-Visible spectroscopy. The biotinylated DNA aptamer sequence specific to the malathion was immobilized onto the iron oxide doped-chitosan/FTO electrode by using streptavidin as linking molecule. Various characterization studies like Field Emission-Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR), and Electrochemical studies were performed to attest the successful fabrication of bioelectrodes. Experimental, parameters like aptamer concentration, response time, stability of electrode and reusability studies were optimized. Aptamer immobilized chitosan-iron oxide nanocomposite (APT/SAICHIT-IO/FTO) bioelectrodes exhibited LOD of about 0.001 ng/mL within 15 min and spike-in studies revealed about 80-92% recovery of malathion from the lettuce leaves and soil sample. (C) 2016 Elsevier B.V. All rights reserved.