Cytokinesis is blocked in mammalian cells transfected with Chlamydia trachomatis gene CT223.
Cytokinesis is blocked in mammalian cells transfected with Chlamydia trachomatis gene CT223.
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DOI：
10.1186/1471-2180-9-2
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发表时间：
2009-01-05
期刊：
影响因子：
4.2
通讯作者：
Rockey, Daniel D
中科院分区：
文献类型：
--
作者：
Alzhanov, Damir T;Weeks, Sara K;Rockey, Daniel D
背景：衣原体改变了宿主细胞生物学的许多方面，包括分裂过程，但这些改变的分子生物学仍然知之甚少。衣原体包涵膜蛋白 (Incs) 可能是与宿主细胞胞质蛋白直接相互作用的候选者，因为它们被分泌到包涵膜并暴露于胞质溶胶。 inc 基因 CT223 是编码或预计编码 Inc 蛋白的一组顺序 orf 之一。在所有测试的沙眼衣原体血清型中，CT223p 均定位于包涵膜。结果：使用质粒转染方法来检查 CT223 和其他 Inc 蛋白产物在未感染的哺乳动物细胞内的功能。使用荧光显微镜证明 CT223 以及在较小程度上相邻的 inc 基因能够阻断宿主细胞胞质分裂并促进其他人在衣原体感染细胞中观察到的着丝粒超数缺陷。两种表型均与编码 CT223p 羧基末端尾部的质粒转染有关，CT223p 是该蛋白的一个区域，可能暴露于感染细胞中的胞质溶胶。结论：这些研究表明，某些 Inc 蛋白可阻断沙眼衣原体感染细胞中的胞质分裂。这些结果与其他人的工作一致，显示衣原体抑制宿主细胞胞质分裂。
BACKGROUND: The chlamydiae alter many aspects of host cell biology, including the division process, but the molecular biology of these alterations remains poorly characterized. Chlamydial inclusion membrane proteins (Incs) are likely candidates for direct interactions with host cell cytosolic proteins, as they are secreted to the inclusion membrane and exposed to the cytosol. The inc gene CT223 is one of a sequential set of orfs that encode or are predicted to encode Inc proteins. CT223p is localized to the inclusion membrane in all tested C. trachomatis serovars.RESULTS: A plasmid transfection approach was used to examine the function of the product of CT223 and other Inc proteins within uninfected mammalian cells. Fluorescence microscopy was used to demonstrate that CT223, and, to a lesser extent, adjacent inc genes, are capable of blocking host cell cytokinesis and facilitating centromere supranumeracy defects seen by others in chlamydiae-infected cells. Both phenotypes were associated with transfection of plasmids encoding the carboxy-terminal tail of CT223p, a region of the protein that is likely exposed to the cytosol in infected cells.CONCLUSION: These studies suggest that certain Inc proteins block cytokinesis in C. trachomatis-infected cells. These results are consistent with the work of others showing chlamydial inhibition of host cell cytokinesis.