Tissue-specific extracellular matrix coatings for the promotion of cell proliferation and maintenance of cell phenotype.
Tissue-specific extracellular matrix coatings for the promotion of cell proliferation and maintenance of cell phenotype.
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DOI：
10.1016/j.biomaterials.2009.04.005
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发表时间：
2009-08
期刊：
影响因子：
14
通讯作者：
Van Dyke M
中科院分区：
文献类型：
--
作者：
Zhang Y;He Y;Bharadwaj S;Hammam N;Carnagey K;Myers R;Atala A;Van Dyke M
最近的研究表明，细胞外基质（ECM）替代品可以对细胞生长，分化和功能产生显着影响。然而，这些ECM通常是通用的，尚未开发用于特定细胞类型。在这项研究中，我们开发了用于皮肤、骨骼肌和肝细胞培养的组织特异性ECM基涂层基质。从成人皮肤、骨骼肌和肝组织中去除细胞组分，并将所得脱细胞基质均质化并溶解。ECM溶液用于包被培养皿。组织匹配和非组织匹配的细胞类型在这些涂层上生长，以在几个时间点评估粘附、增殖、表型维持和细胞功能。每种细胞类型在含有来自其来源组织的ECM的培养物中显示出更好的增殖和分化。虽然在本研究中没有研究三种ECM类型的细微组成差异，但这些结果表明，组织特异性ECM提供了一种培养微环境，当用作涂层基质时，该微环境与体内环境相似，并且这种新的培养技术具有用于药物开发和开发基于细胞的疗法的潜力。
Recent studies have shown that extra-cellular matrix (ECM) substitutes can have a dramatic impact on cell growth, differentiation and function. However, these ECMs are often applied generically and have yet to be developed for specific cell types. In this study, we developed tissue-specific ECM-based coating substrates for skin, skeletal muscle and liver cell culture. Cellular components were removed from adult skin, skeletal muscle, and liver tissues, and the resulting acellular matrices were homogenized and dissolved. The ECM solutions were used to coat culture dishes. Tissue matched and non-tissue matched cell types were grown on these coatings to assess adhesion, proliferation, maintenance of phenotype and cell function at several time points. Each cell type showed better proliferation and differentiation in cultures containing ECM from their tissue of origin. Although subtle compositional differences in the three ECM types were not investigated in this study, these results suggest that tissue specific ECMs provide a culture microenvironment that is similar to the in vivo environment when used as coating substrates, and this new culture technique has the potential for use in drug development and the development cell-based therapies.
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影响因子：
7.8
作者：
SPRAY, DC;GINZBERG, RD;MORALES, EA;GATMAITAN, Z;ARIAS, IM
通讯作者：
ARIAS, IM
影响因子：
4
作者：
Huet, C;Pisselet, C;Monniaux, D
通讯作者：
Monniaux, D
DOI：
10.1007/s00417-004-1116-3
发表时间：
2005-07-01
影响因子：
2.7
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
13.3
作者：
Salber, Jochen;Graeter, Stefan;Moeller, Martin
通讯作者：
Moeller, Martin