Virus adhesion to archetypal fomites: A study with human adenovirus and human respiratory syncytial virus
Virus adhesion to archetypal fomites: A study with human adenovirus and human respiratory syncytial virus
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DOI：
10.1016/j.cej.2021.132085
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发表时间：
2021-08
影响因子：
15.1
通讯作者：
Xunhao Wang;V. Tarabara
中科院分区：
文献类型：
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作者：
Xunhao Wang;V. Tarabara
基于病毒和污染物的物理化学性质，对两种病毒-一种有包膜病毒（人呼吸道合胞病毒，HRSV）和一种无包膜病毒（人腺病毒5，HAdV 5）-与四种污染物（二氧化硅，尼龙，不锈钢，聚丙烯）的粘附进行定量和解释。所选的污染物被初步确定为“原型”，代表组的材料明显不同的机制，他们的界面相互作用。这些表面基于它们的表面能组分而典型化，包括色散（Lifshitz-van der Waals）组分和两个极性（电子供体和电子受体）组分。使用扩展的Derjaguin-Landau-Verwey-Overbeek（XDLVO）理论预测病毒-污染物相互作用，并用石英晶体微天平（QCM-D）进行实验评估。极性相互作用（表现为疏水吸引力的所有病毒污染物对，但HAdV 5/二氧化硅）管理病毒附着污染物从高离子强度的解决方案，典型的呼吸液，而分散的相互作用发挥了相对较小的作用。对于HAdV 5和HRSV，沉积病毒的面积质量密度与水中病毒-污染物界面相互作用的自由能Δ G vwf相关。病毒-污染物附着概率对Δ G vwf的依赖性在HAdV 5和HRSV中均变为一种趋势，表明有可能使用Δ G vwf作为病毒粘附的预测因子。用去离子水冲洗污染物导致部分病毒去除，这归因于较长范围的排斥静电相互作用。所提出的方法可以指导筛选和选择的材料，阻止病毒粘附。关于病毒附着于材料的效率作为其表面能组分的函数的信息可以帮助设计防粘附表面，开发表面清洁解决方案和协议，并为室内环境中的病毒的运输和命运模型提供信息。
Adhesion of two viruses–one enveloped (human respiratory syncytial virus, HRSV) and one non-enveloped (human adenovirus 5, HAdV5)–to four fomites (silica, nylon, stainless steel, polypropylene) was quantified and interpreted based on physicochemical properties of viruses and fomites. The selected fomites are tentatively identified as “archetypes” representing groups of materials distinctly different in mechanisms of their interfacial interactions. The surfaces are typified on the basis of their surface energy components including the dispersive (Lifshitz-van der Waals) component and two polar (electron donor and electron acceptor) components. Virus-fomite interactions are predicted using the extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory and are experimentally assessed in tests with quartz crystal microbalance with dissipation (QCM-D). Polar interactions (manifested as hydrophobic attraction for all virus-fomite pairs but HAdV5/silica) governed virus attachment to fomites from a solution of high ionic strength typical for a respiratory fluid, while dispersive interactions played a relatively minor role. For both HAdV5 and HRSV, the areal mass density of deposited viruses correlated with the free energy of virus-fomite interfacial interaction in water, Δ G vwf. The dependence of virus-fomite attachment probability on Δ G vwf collapsed into one trend for both HAdV5 and HRSV pointing to the possibility of using Δ G vwf as a predictor of virus adhesion. Fomite rinsing with DI water resulted in a partial virus removal attributable to longer range repulsive electrostatic interactions. The proposed methodology can guide screening and selection of materials that discourage virus adhesion. The information on the efficiency of virus attachment to materials as a function of their surface energy components can help design anti-adhesive surfaces, develop surface cleaning solutions and protocols, and inform transport and fate models for viruses in indoor environments.