Transcriptomic, proteomic, and physiological comparative analyses of flooding mitigation of the damage induced by low-temperature stress in direct seeded early indica rice at the seedling stage.
Transcriptomic, proteomic, and physiological comparative analyses of flooding mitigation of the damage induced by low-temperature stress in direct seeded early indica rice at the seedling stage.
复制标题
直播早籼苗期淹水缓解低温胁迫损伤的转录组学、蛋白质组学和生理学比较分析
DOI：
10.1186/s12864-021-07458-9
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发表时间：
2021-03-12
期刊：
影响因子：
4.4
通讯作者：
Zeng Y
中科院分区：
文献类型：
--
作者：
Wang W;Du J;Chen L;Zeng Y;Tan X;Shi Q;Pan X;Wu Z;Zeng Y
背景水稻早育期苗期经常发生低温，特别是直接播种早育期，采用水淹灌溉可以减轻水稻幼苗的低温危害。水驱减轻低温胁迫损伤的分子机制尚未完全阐明。因此，在8°C低温胁迫、低温伴水（LTF）和对照（CK）处理下，建立3天的低温胁迫处理，以确定直接播种稻幼苗期的转录组学、蛋白质组学和生理反应。结果与对照相比，slf对叶绿体和类囊体片层造成损伤，亲渗体和淀粉粒增加，而LTF减轻了LTF对叶绿体结构的损伤。处理植株的生理特征表明，与LT相比，LTF显著提高了rubisco、叶绿素、PEPCK、ATP和ga3的含量，显著降低了可溶性蛋白、MDA和ABA的含量。无4d标记的定量蛋白质组学分析显示，在LT/CK和LTF/CK对照组中，光合反应蛋白（如光敏色素）、叶绿素和三羧酸循环显著下调。但与LT相比，LTF的光敏色素、叶绿素内酯加氧酶活性和葡聚糖分支酶活性在水稻叶片中显著上调。转录组学和蛋白质组学研究鉴定了72,818个转录本和5639个蛋白质，在转录组学和蛋白质组学水平上鉴定了4983个基因。差异表达基因（DEGs）和差异表达蛋白（DEPs）在甘氨酸、丝氨酸和苏氨酸代谢、次生代谢物生物合成、糖酵解/糖异生和代谢途径中显著富集。结论通过转录组学、蛋白质组学和生理学分析，我们确定了LT和LTF诱导了多种代谢途径的变化。GO和KEGG富集分析表明，DEGs和DEPs与光合作用途径、抗氧化酶和能量代谢途径相关蛋白有关。本研究为减少低温胁迫对直接种子水稻的危害提供了新的思路，并为低温抗洪品种的选育提供了新的靶点。进一步分析翻译调控和代谢物可能有助于阐明水淹减轻苗期直接种子早期指标低温胁迫的分子机制。
BackgroundLow temperature (LT) often occurs at the seedling stage in the early rice-growing season, especially for direct seeded early-seasonindicarice, and using flooding irrigation can mitigate LT damage in rice seedlings. The molecular mechanism by which flooding mitigates the damage induced by LT stress has not been fully elucidated. Thus, LT stress at 8 °C, LT accompanied by flooding (LTF) and CK (control) treatments were established for 3 days to determine the transcriptomic, proteomic and physiological response in direct seeded rice seedlings at the seedling stage.ResultsLT damaged chloroplasts, and thylakoid lamellae, and increased osmiophilic bodies and starch grains compared to CK, but LTF alleviated the damage to chloroplast structure caused by LT. The physiological characteristics of treated plants showed that compared with LT, LTF significantly increased the contents of rubisco, chlorophyll, PEPCK, ATP and GA3but significantly decreased soluble protein, MDA and ABA contents. 4D-label-free quantitative proteomic profiling showed that photosynthesis-responsive proteins, such as phytochrome, as well as chlorophyll and the tricarboxylic acid cycle were significantly downregulated in LT/CK and LTF/CK comparison groups. However, compared with LT, phytochrome, chlorophyllide oxygenase activity and the glucan branching enzyme in LTF were significantly upregulated in rice leaves. Transcriptomic and proteomic studies identified 72,818 transcripts and 5639 proteins, and 4983 genes that were identified at both the transcriptome and proteome levels. Differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) were significantly enriched in glycine, serine and threonine metabolism, biosynthesis of secondary metabolites, glycolysis/gluconeogenesis and metabolic pathways.ConclusionThrough transcriptomic, proteomic and physiological analyses, we determined that a variety of metabolic pathway changes were induced by LT and LTF. GO and KEGG enrichment analyses demonstrated that DEGs and DEPs were associated with photosynthesis pathways, antioxidant enzymes and energy metabolism pathway-related proteins. Our study provided new insights for efforts to reduce the damage to direct seeded rice caused by low-temperature stress and provided a breeding target for low temperature flooding-resistant cultivars. Further analysis of translational regulation and metabolites may help to elucidate the molecular mechanisms by which flooding mitigates low-temperature stress in direct seeded earlyindicarice at the seedling stage.