Comparison of extracts and toxicities of organic compounds in drinking water concentrated by single and composite XAD resins.
Comparison of extracts and toxicities of organic compounds in drinking water concentrated by single and composite XAD resins.
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DOI：
10.2166/wh.2013.035
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发表时间：
2013-12
影响因子：
2.3
通讯作者：
Xue Zhou;Lunhui Xiang;Fenghong Wu;Xiaoling Peng;Hong Xie;Jiachun Wang;Ke-di Yang;Wenqing Lu;Zhigang Wu
中科院分区：
文献类型：
--
作者：
Xue Zhou;Lunhui Xiang;Fenghong Wu;Xiaoling Peng;Hong Xie;Jiachun Wang;Ke-di Yang;Wenqing Lu;Zhigang Wu
比较了XAD-2/8复合树脂（等体积混合XAD-2和XAD-8树脂）与单一XAD-2（非极性）和XAD-8（极性）树脂对饮用水中有机物（OC）的提取率和毒性。饮用水采用中国武汉段的汉江和长江原水加工而成。所有树脂的提取效率控制在30%。采用气相色谱-质谱联用仪（GC-MS）检测OCs的种类，MTT法和彗星试验分别检测OCs对人肝癌HepG 2细胞的细胞毒性和遗传毒性。我们的研究结果表明，XAD-2/8提取更大的各种OC，与XAD-8和XAD-2相比。处理24 h后，各浓度OCs的细胞毒性和遗传毒性均为XAD-8> XAD-2/8> XAD-2（P < 0.05）。我们的研究结果表明，单一的XAD树脂选择性地提取极性或非极性的有机碳，这将导致高估或低估的饮用水的毒性。然而，复合树脂萃取极性和非极性的有机碳，并可以利用作为一个有用的提取技术，以评估饮用水中的有机碳的水平和毒性。
We compared extracts and toxicities of organic compounds (OCs) in drinking water concentrated by composite XAD-2/8 resin (mixed with an equal volume of XAD-2 and XAD-8 resins) with those extracted by single XAD-2 (non-polar) and XAD-8 (polar) resins. Drinking water was processed from raw water of the Han River and the Yangtze River in Wuhan section, China. The extraction efficiency of all resins was controlled at 30%. The types of extracted OCs were detected by gas chromatography-mass spectrometry, and the cytotoxicity and genotoxicity were assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and comet assays, respectively, in human hepatoma HepG2 cells. Our results showed that XAD-2/8 extracted a larger variety of OCs, compared with XAD-8 and XAD-2. The cytotoxicity and genotoxicity of extracted OCs were in the order of XAD-8> XAD-2/8> XAD-2 at almost all tested concentrations after 24 h treatment (P < 0.05). Our findings suggest that single XAD resin selectively extracts either polar or non-polar OCs, which would lead to over- or under-estimation of the toxicity of drinking water. Nevertheless, composite resin extracts both polar and non-polar OCs, and could be utilized as a useful extraction technique to evaluate the level and toxicity of OCs in drinking water.