Analyses of five gallinacin genes and the Salmonella enterica serovar Enteritidis response in poultry
Analyses of five gallinacin genes and the Salmonella enterica serovar Enteritidis response in poultry
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DOI：
10.1128/iai.00027-06
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发表时间：
2006-06-01
影响因子：
3.1
通讯作者：
Lamont, Susan J.
中科院分区：
文献类型：
--
作者：
Hasenstein, Jason R.;Zhang, Guolong;Lamont, Susan J.
家禽中的Gallinacins是哺乳动物β-防御素的功能等同物，其构成先天免疫系统的组成部分。肠道沙门氏菌是一种革兰氏阴性菌，对人类和动物健康都有负面影响。分析鸡抗菌素基因的遗传变异与抗沙门氏菌表型反应的关系。通过将四只远交的肉鸡公畜与两个高度近交系的母畜杂交来产生鸡的F-1群体。对F-1代雏鸡接种致病性S.肠道血清型肠炎沙门氏菌接种和接种后的循环抗体水平。肠球菌血清型肠球菌疫苗。研究了五个候选基因，包括鸡毒素2、3、4、5和7。基因片段进行测序的创始人个人的资源人口，并确定了平均13.2单核苷酸多态性（SNP）每个酶。每个基因一个等位基因定义的SNP被用来测试父系等位基因与后代对S.肠道血清型在所评估的5个gallinacin基因中，发现肉鸡公猪中的Gal 3和Gal 7 SNPs与S.肠道血清型肠炎疫苗接种。利用这些SNPs作为分子标记，用于对S.肠道血清型肠炎可导致家禽中免疫应答的增强。
Gallinacins in poultry are functional equivalents of mammalian beta-defensins, which constitute an integral component of the innate immune system. Salmonella enterica serovar Enteritidis is a gram-negative bacterium that negatively affects both human and animal health. To analyze the association of genetic variations of the gallinacin genes with the phenotypic response to S. enterica serovar Enteritidis, an F-1 population of chickens was created by crossing four outbred broiler sires to dams of two highly inbred lines. The F-1 chicks were evaluated for bacterial colonization after pathogenic S. enterica serovar Enteritidis inoculation and for circulating antibody levels after inoculation with S. enterica serovar Enteritidis bacterin vaccine. Five candidate genes were studied, including gallinacins 2, 3, 4, 5, and 7. Gene fragments were sequenced from the founder individuals of the resource population, and a mean of 13.2 single-nucleotide pollymorphisms (SNP) per kilobase was identified. One allele-defining SNP per gene was utilized to test for statistical associations of sire alleles with progeny response to S. enterica serovar Enteritidis. Among the five gallinacin genes evaluated, the Gal3 and Gal7 SNPs in broiler sires were found to be associated with antibody production after S. enterica serovar Enteritidis vaccination. Utilization of these SNPs as molecular markers for the response to S. enterica serovar Enteritidis may result in the enhancement of the immune response in poultry.