Complete nucleotide sequence and gene arrangement of the mitochondrial genome of the crab-eating frog Fejervarya cancrivora and evolutionary implications
Complete nucleotide sequence and gene arrangement of the mitochondrial genome of the crab-eating frog Fejervarya cancrivora and evolutionary implications
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DOI：
10.1016/j.gene.2008.09.010
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发表时间：
2009-07-15
期刊：
影响因子：
3.5
通讯作者：
Zhong, Yang
中科院分区：
文献类型：
--
作者：
Ren, Zhumei;Zhu, Bin;Zhong, Yang
测定了食蟹蛙Fejervarya cancrivora Gravenhorst（两栖纲：无尾目：蛙科）线粒体基因组的全核苷酸序列。mt基因组全长17843 bp，包含13个蛋白编码基因（ATP6、ATP8、COI-III、ND1-6和4L、Cyt b）和2个核糖体RNA基因（12S和16SrRNA）。虽然后生动物mt基因组通常编码22个转移RNA基因（tRNA），但由于存在额外的tRNA拷贝（Met）， E cancrivora mtDNA包含23个tRNA。还发现了一个主要的非编码区和一个突出的基因间间隔区，与控制区和光链复制起源相对应。为了确定F canrivora的系统发育位置，我们将其与其他无尾动物的基因排列进行了比较，并基于mt基因组数据进行了系统发育分析。E cancrivora的mtDNA基因组组织与典型脊椎动物和新蛙不同，但与F limnocharis相同，表明这种独特的基因排列发生在该属的共同祖先身上。系统发育分析支持这里使用的Fejervarya物种的单系性以及双舌犀枝。虽然以前认识到的Ranidae科（=Ranidae, disglossidae，和其他一些natatanuran科；sensu Frost et al., 2006）在最大简约分析中被显示为一个分支，但最大似然和贝叶斯分析表明Ranidae相对于Mantellidae和Rhacophoridae科是一个分支。基因区域的三串联重复以及随后的多余基因缺失被提出来解释额外tRNA（Met）和ND5从原始新巴氏基因序列的易位的进化。(C) 2008年Elsevier B.V.出版
The complete nucleotide sequence of the mitochondrial (mt) genome of the crab-eating frog, Fejervarya cancrivora Gravenhorst (Amphibia: Anura: Ranidae), was determined. The mt genome is 17,843 bp long and contains 13 protein-coding (ATP6, ATP8, COI-III, ND1-6 and 4L, and Cyt b) and two ribosomal RNA (12S and 16SrRNA) genes. Although metazoan mt genomes typically encode 22 transfer RNA genes (tRNAs), the E cancrivora mtDNA contains 23 tRNAs due to the presence of an extra copy of tRNA(Met). A major noncoding region and a prominent intergenic spacer corresponding to the control region and light-strand replication origin were also found. To confirm the phylogenetic position of F cancrivora, we compared the gene arrangement with that of other anurans and performed phylogenetic analyses based on mt genomic data. The genome organization of E cancrivora mtDNA differs from that of typical vertebrates and neobatrachian frogs but is identical with that of F limnocharis, suggesting that the unique gene arrangement occurred in the common ancestor of the genus. Phylogenetic analyses supported the monophyly of the Fejervarya species used here as well as the dicroglossini clade. Although the family Ranidae as previously recognized (=Ranidae, Discoglossidae, and some other natatanuran families; sensu Frost et al., 2006) is shown as a clade in the maximum parsimony analysis, the maximum likelihood and the Bayesian analyses suggest the paraphyly of the Ranidae with respect to the families, Mantellidae and Rhacophoridae. Three-tandem duplications of gene regions followed by subsequent deletions of supernumerary genes were proposed to explain the evolution of the extra tRNA(Met) and translocation of ND5 from the original neobatrachian gene order. (C) 2008 Published by Elsevier B.V.