Hepatitis B virus encodes an RNA polymerase III transcript.
Hepatitis B virus encodes an RNA polymerase III transcript.
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乙型肝炎病毒编码 RNA 聚合酶 III 转录本。
DOI：
10.1128/mcb.3.10.1774-1782.1983
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发表时间：
1983
影响因子：
5.3
通讯作者：
Rutter,WJ
中科院分区：
文献类型：
--
作者：
Standring,DN;Rall,LB;Laub,O;Rutter,WJ
我们证明，克隆的B型肝炎病毒（HBV）DNA指导合成的700个碱基的RNA（HBV 700）的RNA聚合酶III在无细胞转录系统。HBV 700是已知的唯一源自病毒短链的转录物，并已定位于病毒图谱上大约1，635和954个碱基对之间的区域，位于表面和核心抗原编码序列之间，但与推定的DNA聚合酶和B蛋白基因重叠和相对。HBV 700和核心抗原RNA的体外起始位点仅相距50个碱基，表明这两个基因可能是协同调控的。此外，这两个起始位点似乎都位于HBV短链5′端和病毒长链切口之间的约300个碱基的双链区域（切口区域）内。我们发现两个不寻常的序列元素之间的人类和土拨鼠病毒的切口区域是保守的。
We demonstrated that cloned hepatitis B virus (HBV) DNA directs the synthesis of a 700-base RNA (HBV 700) by RNA polymerase III in a cell-free transcription system. HBV 700 is the only transcript known to originate from the viral short strand and has been mapped to the region between roughly 1,635 and 954 base pairs on the viral map, between the surface and core antigen coding sequences but overlapping and opposing the putative DNA polymerase and B protein genes. The in vitro initiation sites for the HBV 700 and core antigen RNAs are only 50 bases apart, suggesting that these two genes may be coordinately regulated. Moreover, both of these initiation sites appear to lie within the ~300-base double-stranded region (the nick region) between the 5′ end of the HBV short strand and the nick in the viral long strand. We found two unusual sequence elements in the nick region that are conserved between the human and woodchuck viruses.