Acid sensitivity of the spinal dorsal root ganglia C-fiber nociceptors innervating the guinea pig esophagus.
Acid sensitivity of the spinal dorsal root ganglia C-fiber nociceptors innervating the guinea pig esophagus.
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DOI：
10.1111/nmo.12561
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发表时间：
2015-06
影响因子：
3.5
通讯作者：
Kollarik M
中科院分区：
文献类型：
--
作者：
Ru F;Banovcin P Jr;Kollarik M
Gastroesophageal reflux can cause high acidity in the esophagus and trigger heartburn and pain. However, because of the esophageal mucosal barrier, the acidity at the nerve terminals of pain-mediating C-fibers in esophageal mucosa is predicted to be substantially lower. We hypothesized that the esophageal DRG C-fibers are activated by mild acid (compared to acidic reflux), and express receptors and ion channels highly sensitive to acid. Extracellular single unit recordings of activity originating in esophageal DRG C-fiber nerve terminals were performed in the innervated esophagus preparation ex vivo. Acid was delivered in a manner that bypassed the esophageal mucosal barrier. The expression of mRNA for selected receptors in esophagus-specific DRG neurons was evaluated using single cell RT-PCR. Mild acid (pH=6.5–5.5) activated esophageal DRG C-fibers in a pH-dependent manner. The response to mild acid at pH=6 was not affected by the TRPV1 selective antagonist iodo-resiniferatoxin. The majority (70–95%) of esophageal DRG C-fiber neurons (TRPV1-positive) expressed mRNA for acid sensing ion channels (ASIC1a, ASIC1b, ASIC2b and/or ASIC3), two-pore-domain (K2P) potassium channel TASK1, and the proton-sensing G-protein coupled receptor OGR1. Other evaluated targets (PKD2L1, TRPV4, TASK3, TALK1, G2A, GPR4 and TDAG8) were expressed rarely. Guinea pig esophageal DRG C-fibers are activated by mild acid via a TRPV1-independent mechanism, and express mRNA for several receptors and ion channels highly sensitive to acid. The high acid sensitivity of esophageal C-fibers may contribute to heartburn and pain in conditions of reduced mucosal barrier function.
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DOI：
10.1152/ajpgi.00129.2014
发表时间：
2014-11-01
影响因子：
4.5
作者：
Dusenkova, Svetlana;Ru, Fei;Kollarik, Marian
通讯作者：
Kollarik, Marian
影响因子：
5.5
作者：
Kollarik, M;Undem, BJ
通讯作者：
Undem, BJ
影响因子：
3.5
作者：
Huang, Chia-Wei;Tzeng, Jian-Ning;Sun, Wei-Hsin
通讯作者：
Sun, Wei-Hsin
影响因子：
4.8
作者：
Lopes, CMB;Gallagher, PG;Goldstein, SAN
通讯作者：
Goldstein, SAN
影响因子：
3.3
作者：
Leffler, A;Mönter, B;Koltzenburg, M
通讯作者：
Koltzenburg, M