Hemodialysis does not influence the peroxidative state already present in uremia
Hemodialysis does not influence the peroxidative state already present in uremia
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DOI：
10.1159/000045831
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发表时间：
2000-12-01
期刊：
影响因子：
2.5
通讯作者：
Koyama, A
中科院分区：
文献类型：
--
作者：
Hirayama, A;Nagase, S;Koyama, A
血液透析（HD）患者暴露于高氧化应激，然而，这种应激的性质尚不清楚。在这项研究中，我们采用了一种特殊的脂质过氧化产物，磷脂酰胆碱氢过氧化物（PCOOH），并通过测量血浆和红细胞膜中的硫代巴比妥酸反应物质（TBARS）和PCOOH来评估终末期肾病的过氧化作用。我们还调查了HD治疗前后血浆TBARS和PCOOH，从而通过单一HD手术评估氧化应激。健康对照组血浆TBARS水平为2.9 +/- 0.4 nmol/ml。HD患者在HD治疗前后的TBARS水平分别为5.1 +/- 1.4和3.1 +/- 0.5 nmol/ml，且HD前血浆TBARS水平显著高于对照组和HD后。HD前患者血浆PCOOH浓度为119.7 +/- 58.4 pmol/ml，显著高于对照组的88.6 +/- 14.3 pmol/ml。HD后血浆PCOOH水平降至103.2 +/- 36.0 pmol/ml，仍显著高于对照组。在红细胞方面，患者血浆PCOOH为259.3 +/- 105.4 nmol/g RBC，明显高于对照组的88.6 +/- 32.0 nmol/g REC。从肾脏疾病的病因分析，多囊肾病患者血浆PCOOH水平明显低于其他患者。这些结果表明，HD患者血浆和红细胞中的脂质过氧化都有所增加，尽管这种氧化应激不是由HD引起的。版权所有(C) 2000 S. Karger AG，巴塞尔。
Hemodialysis (HD) patients are exposed to high oxidative stress, however, the nature of this stress is still unclear. In this study, we employed a specific lipid peroxidative product, phosphatidylcholine hydroperoxide (PCOOH), and evaluated the peroxidative effect of end stage renal disease by measuring thiobarbituric acid reactive substances (TBARS) and PCOOH in both plasma and erythrocyte membrane. We also surveyed plasma TBARS and PCOOH before and after HD sessions thereby assessing oxidative stress by a single HD procedure. The plasma TBARS level of healthy controls was 2.9 +/- 0.4 nmol/ml. Those of HD patients before and after HD session were 5.1 +/- 1.4 and 3.1 +/- 0.5 nmol/ml, respectively, and the pre-HD plasma TBARS levels were significantly higher than those of controls and after HD. The Plasma PCOOH concentration of patients before HD was 119.7 +/- 58.4 pmol/ml and was significantly higher than that of controls which was 88.6 +/- 14.3 pmol/ml. After HD, the plasma PCOOH level decreased to 103.2 +/- 36.0 pmol/ml, which was still significantly higher than that of controls. In erythrocytes, the PCOOH level of patients was 259.3 +/- 105.4 nmol/g RBC and was significantly higher than that of controls with 88.6 +/- 32.0 nmol/g REC. Analyzed with respect to the cause of renal disease, the polycystic kidney disease patients showed significantly lower plasma PCOOH levels than the others. These results suggest that there is an increase of lipid peroxidation in both plasma and erythrocytes of HD patients, though this oxidative stress was not brought about by HD. Copyright (C) 2000 S. Karger AG, Basel.