GRGDSP peptide-bound silicone membranes withstand mechanical flexing in vitro and display enhanced fibroblast adhesion.
GRGDSP peptide-bound silicone membranes withstand mechanical flexing in vitro and display enhanced fibroblast adhesion.
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DOI：
10.1016/s0142-9612(02)00062-5
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发表时间：
2002-08
期刊：
影响因子：
14
通讯作者：
S. S. Lateef-S.;S. Boateng;Thomas J. Hartman;C. Crot;B. Russell;L. Hanley
中科院分区：
文献类型：
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作者：
S. S. Lateef-S.;S. Boateng;Thomas J. Hartman;C. Crot;B. Russell;L. Hanley
心脏组织的机械生物学研究需要能够在体外对细胞施加力的装置。有机硅弹性体经常用于这些设备中，因为它具有柔韧性和透明性，可以对细胞进行光学成像。然而，未经处理的天然有机硅具有疏水性，不适合细胞培养。这里检查了共价结合到有机硅表面的肽在体外动态弯曲过程中增强细胞粘附的情况。描述了用共价结合的 GRGDSP 肽对医用级有机硅膜进行化学修饰的程序。然后重复心脏细胞培养物的机械研究条件，并证明肽层在体外机械弯曲后可存活 48 小时。具体而言，30pmol/cm2 肽修饰的有机硅膜的体外机械弯曲对保持与表面结合的肽的量没有显着影响。与天然有机硅或组织培养聚苯乙烯相比，心脏成纤维细胞在生长至少 24 小时内对这些肽结合有机硅膜的粘附力增强。还检查了血清与无血清培养基对成纤维细胞生长的影响。
Mechanobiological studies of cardiac tissue require devices that allow forces to be exerted on cells in vitro. Silicone elastomer is often used in these devices because it is flexible and transparent, permitting optical imaging of the cells. However, native untreated silicone is hydrophobic and is unsuitable for cell culture. Peptides covalently bound to silicone surfaces are examined here for the enhancement of cellular adhesion during in vitro dynamic flexing. A procedure is described for the chemical modification of medical grade silicone membranes with covalently bound GRGDSP peptides. The conditions for mechanical studies of cardiac cell cultures are then duplicated and it is demonstrated that the peptide layers survive 48h of mechanical flexing in vitro. Specifically, mechanical flexing in vitro of the 30pmol/cm2peptide-modified silicone membranes has no significant effect on the amount of peptides that remains bound to the surface. Cardiac fibroblasts display enhanced adhesion to these peptide-bound silicone membranes for at least 24h of growth, compared with native silicone or tissue culture polystyrene. The effects of serum versus serum-free media on fibroblast growth are also examined.