Activation of JNK/SAPK in primary glial cultures: II. Differential activation of kinase isoforms corresponds to their differential expression.
Activation of JNK/SAPK in primary glial cultures: II. Differential activation of kinase isoforms corresponds to their differential expression.
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原代胶质细胞培养中 JNK/SAPK 的激活：II。
DOI：
10.1023/a:1022489127107
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发表时间：
1998
影响因子：
4.4
通讯作者：
Bhat,NR
中科院分区：
文献类型：
--
作者：
Zhang,P;Hogan,EL;Bhat,NR
最近，我们报道了原代神经胶质细胞中c-Jun氨基末端激酶(JNK)的激活，注意到星形胶质细胞和少突胶质细胞中的激酶激活模式有一定的不同(Zhang等，J Neurosci Res46：114-121；1996)。在这项扩展的研究中，我们检测了JNK1和JNK2亚型在不同类型的胶质细胞中的激活和表达水平，包括体外定义的两种星形胶质细胞亚型(类型1和类型2)、少突胶质细胞和小胶质细胞。细胞抽提物和JNK免疫沉淀物的凝胶内激酶分析表明，在1型星形胶质细胞中，JNK1和JNK2均被肿瘤坏死因子α激活，而在小胶质细胞中，JNK1和JNK2被肿瘤坏死因子α和细菌脂多糖激活。在1型星形胶质细胞和小胶质细胞中两种JNK亚型的强烈激活与在2型星形胶质细胞和少突胶质细胞中JNK1和JNK2的激活形成鲜明对比，这两种胶质细胞亚型具有共同的谱系。免疫印迹和免疫细胞化学分析表明，这两种异构体在不同的神经胶质细胞中有不同的表达，从而解释了观察到的不同激活。
Recently, we reported on the activation of c-Jun N-terminal kinase (JNK) in primary glial cells noting certain differences in the patterns of kinase activation in astrocytes and oligodendrocytes (Zhang et al.,J Neurosci Res46:114–121;1996). In this extended study, we have examined the activation and expression levels of JNK1 and JNK2 isoforms in different glial cell types including the two in vitro-defined astroglial subtypes (type-1 and type-2), oligodendrocytes and microglia. An in-gel kinase assay of cell extracts and JNK-immunoprecipitates revealed the activation of both JNK1 and JNK2 in type-1 astrocytes in response to TNFα, and in microglia, in response to TNFα and bacterial lipopolysaccharide. The strong activation of the two JNK isoforms in type-1 astrocytes and microglia contrasted with a predominant activation of JNK1 over JNK2 in type-2 astrocytes and oligodendrocytes, the two glial subtypes sharing a common lineage. Immunoblot and immunocytochemical analyses using isoform-speciflc antibodies showed a differential expression of the two isoforms in different glial cells thereby accounting for their observed differential activation.