In vivo forces used to develop design parameters for tissue engineered implants for rabbit patellar tendon repair
In vivo forces used to develop design parameters for tissue engineered implants for rabbit patellar tendon repair
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DOI：
10.1016/s0021-9290(02)00459-1
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发表时间：
2003-04-01
影响因子：
2.4
通讯作者：
Butler, DL
中科院分区：
文献类型：
--
作者：
Juncosa, N;West, JR;Butler, DL
以前的组织工程研究已经表明，将未分化的间充质干细胞悬浮在胶原凝胶中并将其包裹在缝线上会导致细胞排列和培养物中的结构收缩，从而形成适合植入肌腱修复的形式。关于这些可能改善肌腱修复生物力学的体内信号的模式知之甚少。本研究使用家兔髌腱（PT）模型检验了三个假设：（1）体内峰值力以及这些力的上升和下降速率将随着活动水平的增加而显著增加;（2）所有活动的PT安全系数将在肌腱的数值范围内（2.5-3）;（3）家兔在评估时不会“偏爱”手术肢体，但在安静站立（QS）期间，两个肢体保持相似的垂直地面反作用力。在QS期间和动物在控制速度（0.04和0.13 m/s）和倾斜度（0 °和12 °）的跑步机上跳跃时，测量在体兔PT力。通过手术将植入式力传感器放置在一个PT中，并在术后3天收集8只新西兰白色家兔的数据。最大张力随跑步机倾斜度的增加而显著增加，肌腱力的上升和下降率随速度和倾斜度的增加而显著增加（p < 0.001）。这样的设计标准应该是有用的机械刺激肌腱修复细胞凝胶结构。(C)2003爱思唯尔科技有限公司版权所有。
Previous studies in tissue engineering have shown that suspending undifferentiated mesenchymal stem cells in collagen gels and wrapping them about a suture causes alignment of cells and contraction of constructs in culture in a form that is suitable for implantation for tendon repair. Little is known about the patterns of these in vivo signals that might improve tendon repair biomechanics. Three hypotheses were tested in this study using the rabbit patellar tendon (PT) model: (1) peak in vivo forces and the rates of rise and fall in these forces will increase significantly with increasing levels of activity; (2) the PTs safety factor for all activities will be in the range of values found for tendons (2.5-3); (3) rabbits will not "favor" the operated limb at the time of evaluation but maintain similar vertical ground reaction forces in both limbs during quiet standing (QS). In vivo rabbit PT forces were measured during QS and while the animal hopped on a treadmill whose speed (0.04 and 0.13 m/s) and inclination (0degrees and 12degrees) were controlled. Implantable force transducers were surgically placed in one PT and data collected three days post surgery in each of eight New Zealand White rabbits. Peak tensile forces increased significantly with inclination of the treadmill and the rates of rise and fall in tendon force increased significantly with both speed and inclination (p < 0.001). Such design criteria should be useful in mechanically stimulating cell-gel constructs for tendon repair. (C) 2003 Elsevier Science Ltd. All rights reserved.