A Mycochemical Investigation of the Black Lingzhi Mushroom, Amauroderma rugosum (Agaricomycetes), Reveals Several Lipidic Compounds with Anti-Inflammatory and Antiproliferative Activities
A Mycochemical Investigation of the Black Lingzhi Mushroom, Amauroderma rugosum (Agaricomycetes), Reveals Several Lipidic Compounds with Anti-Inflammatory and Antiproliferative Activities
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对黑灵芝蘑菇（伞形孢菌）的真菌化学研究揭示了几种具有抗炎和抗增殖活性的脂质化合物
DOI：
10.1615/intjmedmushrooms.2021037977
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发表时间：
2021-01-01
影响因子：
1.2
通讯作者：
Xie, Yizhen
中科院分区：
文献类型：
--
作者：
Chen, Shaodan;Zhang, Yifan;Xie, Yizhen
对药用真菌皱皮天皮伞（Amaurodernta rugosum）进行了真菌化学研究，共分离得到30个化合物，其中甾醇类14个，酚类6个，不饱和脂肪酸类5个，其它5个。这些化合物的结构通过核磁共振光谱和质谱数据与文献数据的比较进行了鉴定。其中，化合物27为新天然产物，化合物2-4、7-13、15-30为首次从该属植物中分离得到。硬脂酸和不饱和脂肪酸在体外表现出抗炎和抗增殖活性。化合物5和6对脂多糖诱导的鼠巨噬细胞RAW264.7细胞中的一氧化氮产生显示出最高的抑制作用，半最大抑制浓度（IC 50）值分别为27.6 +/-2.1 μ M和15.3 +/-2.0 μ M。化合物17对HepG 2和MDA-MB-231细胞系表现出最强的抑制作用，IC 50值< 25 μ M。本研究不仅丰富了对紫茎泽兰化学成分多样性的认识，而且对紫茎泽兰的化学成分多样性的研究也具有重要意义。同时也为进一步开发利用毛茛提供了依据。Rugosum作为新的潜在的抗肿瘤或抗炎化疗剂的来源。
A mycochemical investigation on the medicinal mushroom Amaurodernta rugosum led to the isolation of 30 compounds, including 14 sterols, 6 phenolic constituents, 5 unsaturated fatty acids, and 5 other compounds. The structures of these compounds were elucidated by comparison of their nuclear magnetic resonance spectroscopic and mass spectrometry data with literature data. Among them, compound 27 was obtained as a new natural compound, and compounds 2-4, 7-13, and 15-30 were isolated from the genus Amauroderma for the first time. Sterols and unsaturated fatty acids showed anti-inflammatory and antiproliferative activities in vitro. Compounds 5 and 6 showed the highest inhibitory effect on nitric oxide production in lipopolysaccharide-induced murine macrophage RAW264.7 cells, with half maximal inhibitory concentration (IC50) values of 27.6 +/- 2.1 mu M and 15.3 +/- 2.0 mu M respectively. Compound 17 exhibited the strongest inhibition against HepG2 and MDA-MB-231 cell lines, with IC50 values < 25 mu M. This study not only enriches the understanding of the diversity of chemical constituents in A. rugosum, but it also provides a basis for further development and utilization of A. rugosum as a source of new potential antitumor or anti-inflammatory chemotherapy agents.