A multi‐study analysis of gut microbiome data from the blue mussel ( Mytilus edulis ) emphasises the impact of depuration on biological interpretation
A multi‐study analysis of gut microbiome data from the blue mussel ( Mytilus edulis ) emphasises the impact of depuration on biological interpretation
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对蓝贻贝 (Mytilus edulis) 肠道微生物组数据的多项研究分析强调了净化对生物学解释的影响
DOI：
10.1111/1462-2920.16537
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发表时间：
2023
影响因子：
5.1
通讯作者：
Ward, J. Evan
中科院分区：
文献类型：
--
作者：
Griffin, Tyler W.;Darsan, Mya A.;Collins, Hannah I.;Holohan, Bridget A.;Pierce, Melissa L.;Ward, J. Evan
蓝贻贝（Mytilus edulis）是一种悬浮饲料，已用于肠道微生物组调查。虽然原始的16S序列数据通常是公开的，但缺乏统一的二级分析。目前的工作分析了一个小组在7年内进行的7个项目的原始数据。尽管每个项目都有不同的动机、实验设计和结论，但所有选择的样本都来自M。在长岛海峡的一个地方选出的。该分析的目的是确定哪些独立因素（例如，收集日期、净化状态）负责管理肠道微生物组的组成和多样性。结果表明，贻贝是否经历了净化，这里定义为在受控的无食物时期排泄粪便，是控制肠道微生物组组成的主要因素。来自未净化贻贝的肠道微生物组是居民和临时社区的混合物，并受到时间因素的影响。居民社区从净化贻贝的影响，最终的食物来源和宿主贻贝的时间长度在实验室条件下举行。这些发现强化了肠道微生物群分为常驻和瞬态组分的范式，并建议在设计和解释未来实验时应考虑净化状态。
The blue mussel (Mytilus edulis) is a suspension feeder which has been used in gut‐microbiome surveys. Although raw 16S sequence data are often publicly available, unifying secondary analyses are lacking. The present work analysed raw data from seven projects conducted by one group over 7 years. Although each project had different motivations, experimental designs and conclusions, all selected samples were from the guts ofM. eduliscollected from a single location in Long Island Sound. The goal of this analysis was to determine which independent factors (e.g., collection date, depuration status) were responsible for governing composition and diversity in the gut microbiomes. Results indicated that whether mussels had undergone depuration, defined here as voidance of faeces in a controlled, no‐food period, was the primary factor that governed gut microbiome composition. Gut microbiomes from non‐depurated mussels were mixtures of resident and transient communities and were influenced by temporal factors. Resident communities from depurated mussels were influenced by the final food source and length of time host mussels were held under laboratory conditions. These findings reinforce the paradigm that gut microbiota are divided into resident and transient components and suggest that depuration status should be taken into consideration when designing and interpreting future experiments.