A possible role of arachidonate metabolism in the mechanism of prolactin release.
A possible role of arachidonate metabolism in the mechanism of prolactin release.
复制标题
花生四烯酸代谢在催乳素释放机制中的可能作用。
DOI：
10.1152/ajpendo.1986.250.3.e288
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
MacLeod,RM
中科院分区：
文献类型：
--
作者：
Judd,AM;Koike,K;MacLeod,RM
花生四烯酸从垂体磷脂中裂解可能有助于调节催乳素释放的过程。为了验证这一假设，从雌性大鼠的垂体前叶细胞的原代培养物预孵育与[3 H]花生四烯酸标记其含磷脂成分。然后洗涤细胞并与载体或测试试剂一起孵育，并测量从磷脂裂解的催乳素和[3 H]花生四烯酸释放到培养基中。促甲状腺激素释放激素（TRH）和神经降压素显着增加释放的[3 H]花生四烯酸和催乳素。虽然基础[3 H]花生四烯酸释放不受多巴胺或生长抑素，这两种药物减少[3 H]花生四烯酸释放TRH诱导。通过将细胞暴露于改变钙平衡的试剂来研究钙动员和花生四烯酸释放之间的关系。钙离子通道激活剂-相反，钴，钙通道阻滞剂，五氟利多，钙结合蛋白抑制剂，和低钙介质降低基础和TRH诱导的催乳素释放和减少TRH诱导的花生四烯酸释放。RHC 80267，一种甘油二酯脂肪酶抑制剂，减少TRH诱导的催乳素和花生四烯酸的释放。BW 755 c是花生四烯酸转化为其代谢产物的抑制剂，可降低TRH诱导的催乳素释放，但可预测地增加花生四烯酸释放。这些发现支持花生四烯酸代谢物可能参与调节催乳素释放的过程的假设。
The cleavage of arachidonate from pituitary phospholipids may contribute to the process that regulates the release of prolactin. To test this hypothesis, primary cultures of anterior pituitary cells from female rats were preincubated with [3H]arachidonate to label their phospholipid-containing components. The cells were then washed and incubated with vehicle or test agents and the release into the medium of prolactin and [3H]arachidonate cleaved from the phospholipids was measured. Thyrotropin-releasing hormone (TRH) and neurotensin significantly increased the release of both [3H]arachidonate and prolactin. Although basal [3H]arachidonate release was not affected by dopamine or somatostatin, both of these agents reduced [3H]arachidonate release induced by TRH. The relationship between calcium mobilization and arachidonate release was investigated by exposing the cells to agents that modify calcium balance. Maitotoxin, a calcium channel activator, stimulated prolactin and arachidonate release. In contrast cobalt, a calcium channel blocker, penfluridol, a calcium-binding protein inhibitor, and low-calcium medium decreased basal and TRH-induced prolactin release and diminished the TRH-induced release of arachidonate. RHC 80267, an inhibitor of diacylglycerol lipase, decreased TRH-induced prolactin and arachidonate release. BW755c, an inhibitor of the conversion of arachidonate to its metabolites, decreased TRH-induced prolactin release but predictably increased arachidonate release. These findings support the hypothesis that arachidonate metabolites may be involved in the process regulating prolactin release.