DETECTION OF THE ANTI-HU ANTIBODY IN THE SERUM OF PATIENTS WITH SMALL-CELL LUNG-CANCER - A QUANTITATIVE WESTERN-BLOT-ANALYSIS
DETECTION OF THE ANTI-HU ANTIBODY IN THE SERUM OF PATIENTS WITH SMALL-CELL LUNG-CANCER - A QUANTITATIVE WESTERN-BLOT-ANALYSIS
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DOI：
10.1002/ana.410270515
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发表时间：
1990-05-01
影响因子：
11.2
通讯作者：
POSNER, JB
中科院分区：
文献类型：
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作者：
DALMAU, J;FURNEAUX, HM;POSNER, JB
我们在50名正常人的血清中寻找抗hu抗体的存在；小细胞肺癌44例，未伴有副肿瘤病变；25例伴有副肿瘤感觉神经病变、脑脊髓炎或两者兼有的小细胞肺癌患者。采用亲和素-生物素免疫过氧化物酶法和高灵敏定量Western blot分析，50例正常人血清中未检出抗hu抗体。44例无副肿瘤综合征的小细胞肺癌患者中，7例Western blot检测到抗hu抗体水平（平均滴度为76 U/ml）。这些水平明显低于25例小细胞肺癌和副肿瘤感觉神经病变或脑脊髓炎患者的平均滴度（平均滴度，4,592 U/ml）。非副肿瘤性小细胞肺癌（低抗hu滴度）组以女性为主（5女2男），所有患者在诊断时均为“有限”疾病。在抗体阴性组中，男女比例为16比21,51%的患者患有“广泛”疾病。截至撰写本文时，7例低滴度抗hu抗体患者均未发生副肿瘤综合征。抗hu抗体出现时，是小细胞肺癌的良好标记，当高滴度出现时，是与副肿瘤综合征相关的小细胞肺癌的良好标记。
We looked for the presence of the anti-Hu antibody in the sera from 50 normal subjects; 44 patients with small cell lung cancer, not associated with paraneoplastic disease; and 25 patients with small cell lung cancer associated with paraneoplastic sensory neuropathy, encephalomyelitis, or both. Using the avidin-biotin immunoperoxidase method and a highly sensitive quantitative Western blot analysis, the anti-Hu antibody was not detected in the 50 normal hnuman sera. Seven of the 44 patients with small cell lung cancer but no paraneoplastic syndrome had detectable levels (average titer, 76 U/ml) of anti-Hu antibody on Western blot. These levels are significantly lower than the average titer of the 25 patients who had small cell lung cancer and paraneoplastic sensory neuropathy or encephalomyelitis (average titer, 4,592 U/ml). In the group with nonparaneoplastic small cell lung cancer (low anti-Hu titer) there was a predominance of women (5 women: 2 men), and all patients had "limited" disease when diagnosed. In the antibody-negative group the sex ratio was 16 women to 21 men and 51% of the patients had "extensive" disease. None of the 7 patients with a low-titer anti-Hu antibody developed a paraneoplastic syndrome by the time of writing. The anti-Hu antibody appears, when present, to be a good marker for small cell lung cancer and, when present at high titer, for small call lung cancer associated with a paraneoplastic syndrome.